Show simple item record

dc.contributor.authorCrouzier, T.
dc.contributor.authorJang, H.
dc.contributor.authorAhn, J.
dc.contributor.authorStocker, Roman
dc.contributor.authorRibbeck, Katharina
dc.date.accessioned2014-09-15T19:21:32Z
dc.date.available2014-09-15T19:21:32Z
dc.date.issued2013-09
dc.date.submitted2013-06
dc.identifier.issn1525-7797
dc.identifier.issn1526-4602
dc.identifier.urihttp://hdl.handle.net/1721.1/89634
dc.description.abstractThe precise spatial control of cell adhesion to surfaces is an endeavor that has enabled discoveries in cell biology and new possibilities in tissue engineering. The generation of cell-repellent surfaces currently requires advanced chemistry techniques and could be simplified. Here we show that mucins, glycoproteins of high structural and chemical complexity, spontaneously adsorb on hydrophobic substrates to form coatings that prevent the surface adhesion of mammalian epithelial cells, fibroblasts, and myoblasts. These mucin coatings can be patterned with micrometer precision using a microfluidic device, and are stable enough to support myoblast differentiation over seven days. Moreover, our data indicate that the cell-repellent effect is dependent on mucin-associated glycans because their removal results in a loss of effective cell-repulsion. Last, we show that a critical surface density of mucins, which is required to achieve cell-repulsion, is efficiently obtained on hydrophobic surfaces, but not on hydrophilic glass surfaces. However, this limitation can be overcome by coating glass with hydrophobic fluorosilane. We conclude that mucin biopolymers are attractive candidates to control cell adhesion on surfaces.en_US
dc.description.sponsorshipEuropean Commission (Marie Curie International Outgoing Fellowship for Career Development, “BIOMUC”)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH Grant 1R01GM100473)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (award number DMR-819762)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (NSF Grant OCE-0744641-CAREER)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Award DMR-0819762)en_US
dc.description.sponsorshipSamsung Scholarship Foundationen_US
dc.description.sponsorshipMassachusetts Institute of Technology (Startup funds)en_US
dc.description.sponsorshipMassachusetts Institute of Technology (Junior Faculty award)en_US
dc.language.isoen_US
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/bm400447zen_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleCell Patterning with Mucin Biopolymersen_US
dc.typeArticleen_US
dc.identifier.citationCrouzier, T., H. Jang, J. Ahn, R. Stocker, and K. Ribbeck. “Cell Patterning with Mucin Biopolymers.” Biomacromolecules 14, no. 9 (September 9, 2013): 3010–3016.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.contributor.mitauthorStocker, Romanen_US
dc.contributor.mitauthorRibbeck, Katharinaen_US
dc.contributor.mitauthorCrouzier, T.en_US
dc.contributor.mitauthorJang, H.en_US
dc.contributor.mitauthorAhn, J.en_US
dc.relation.journalBiomacromoleculesen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsCrouzier, T.; Jang, H.; Ahn, J.; Stocker, R.; Ribbeck, K.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-8260-338X
dc.identifier.orcidhttps://orcid.org/0000-0002-3199-0508
mit.licenseOPEN_ACCESS_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record