Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix
Author(s)Birjiniuk, Alona; Nance, Elizabeth; Hanes, Justin; Ribbeck, Katharina; Billings, Amanda Nicole; Doyle, Patrick S.; ... Show more Show less
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Biofilms are communities of surface-adherent bacteria surrounded by secreted polymers known as the extracellular polymeric substance. Biofilms are harmful in many industries, and thus it is of great interest to understand their mechanical properties and structure to determine ways to destabilize them. By performing single particle tracking with beads of varying surface functionalization it was found that charge interactions play a key role in mediating mobility within biofilms. With a combination of single particle tracking and microrheological concepts, it was found that Escherichia coli biofilms display height dependent charge density that evolves over time. Statistical analyses of bead trajectories and confocal microscopy showed inter-connecting micron scale channels that penetrate throughout the biofilm, which may be important for nutrient transfer through the system. This methodology provides significant insight into a particular biofilm system and can be applied to many others to provide comparisons of biofilm structure. The elucidation of structure provides evidence for the permeability of biofilms to microscale objects, and the ability of a biofilm to mature and change properties over time.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemical Engineering
New Journal of Physics
Birjiniuk, Alona, Nicole Billings, Elizabeth Nance, Justin Hanes, Katharina Ribbeck, and Patrick S Doyle. “ Single Particle Tracking Reveals Spatial and Dynamic Organization of the Escherichia Coli Biofilm Matrix .” New Journal of Physics 16, no. 8 (August 1, 2014): 085014. © 2014 IOP Publishing Ltd and Deutsche Physikalische Gesellschaft
Final published version