dc.contributor.author | Yu, Ming | |
dc.contributor.author | Mazor, Tali | |
dc.contributor.author | Huang, Hui | |
dc.contributor.author | Huang, Hsuan-Ting | |
dc.contributor.author | Kathrein, Katie L. | |
dc.contributor.author | Woo, Andrew J. | |
dc.contributor.author | Chouinard, Candace R. | |
dc.contributor.author | Labadorf, Adam | |
dc.contributor.author | Akie, Thomas E. | |
dc.contributor.author | Moran, Tyler B. | |
dc.contributor.author | Xie, Huafeng | |
dc.contributor.author | Zacharek, Sima | |
dc.contributor.author | Taniuchi, Ichiro | |
dc.contributor.author | Roeder, Robert G. | |
dc.contributor.author | Kim, Carla F. | |
dc.contributor.author | Zon, Leonard I. | |
dc.contributor.author | Fraenkel, Ernest | |
dc.contributor.author | Cantor, Alan B. | |
dc.date.accessioned | 2014-11-12T13:07:12Z | |
dc.date.available | 2014-11-12T13:07:12Z | |
dc.date.issued | 2012-02 | |
dc.date.submitted | 2011-09 | |
dc.identifier.issn | 10972765 | |
dc.identifier.issn | 1097-4164 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/91518 | |
dc.description.abstract | Polycomb repressive complexes (PRCs) play key roles in developmental epigenetic regulation. Yet the mechanisms that target PRCs to specific loci in mammalian cells remain incompletely understood. In this study we show that Bmi1, a core component of Polycomb Repressive Complex 1 (PRC1), binds directly to the Runx1/CBFβ transcription factor complex. Genome-wide studies in megakaryocytic cells demonstrate significant chromatin occupancy overlap between the PRC1 core component Ring1b and Runx1/CBFβ and functional regulation of a considerable fraction of commonly bound genes. Bmi1/Ring1b and Runx1/CBFβ deficiencies generate partial phenocopies of one another in vivo. We also show that Ring1b occupies key Runx1 binding sites in primary murine thymocytes and that this occurs via PRC2-independent mechanisms. Genetic depletion of Runx1 results in reduced Ring1b binding at these sites in vivo. These findings provide evidence for site-specific PRC1 chromatin recruitment by core binding transcription factors in mammalian cells. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant U54-CA112967) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant R01-GM089903) | en_US |
dc.description.sponsorship | National Science Foundation (U.S.) (Award DB1-0821391) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (P30-ES002109) | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/j.molcel.2011.11.032 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | Elsevier | en_US |
dc.title | Direct Recruitment of Polycomb Repressive Complex 1 to Chromatin by Core Binding Transcription Factors | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Yu, Ming, Tali Mazor, Hui Huang, Hsuan-Ting Huang, Katie L. Kathrein, Andrew J. Woo, Candace R. Chouinard, et al. “Direct Recruitment of Polycomb Repressive Complex 1 to Chromatin by Core Binding Transcription Factors.” Molecular Cell 45, no. 3 (February 2012): 330–343. © 2012 Elsevier Inc. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.mitauthor | Mazor, Tali | en_US |
dc.contributor.mitauthor | Chouinard, Candace R. | en_US |
dc.contributor.mitauthor | Labadorf, Adam | en_US |
dc.contributor.mitauthor | Fraenkel, Ernest | en_US |
dc.relation.journal | Molecular Cell | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Yu, Ming; Mazor, Tali; Huang, Hui; Huang, Hsuan-Ting; Kathrein, Katie L.; Woo, Andrew J.; Chouinard, Candace R.; Labadorf, Adam; Akie, Thomas E.; Moran, Tyler B.; Xie, Huafeng; Zacharek, Sima; Taniuchi, Ichiro; Roeder, Robert G.; Kim, Carla F.; Zon, Leonard I.; Fraenkel, Ernest; Cantor, Alan B. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-9249-8181 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |