Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes
Author(s)Stains, Cliff I.; Tedford, Nathan C.; Walkup, Traci C.; Lukovic, Elvedin; Goguen, Brenda N.; Griffith, Linda G.; Lauffenburger, Douglas A.; Imperiali, Barbara; ... Show more Show less
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Protein kinases catalyze protein phosphorylation and thereby control the flow of information through signaling cascades. Currently available methods for concomitant assessment of the enzymatic activities of multiple kinases in complex biological samples rely on indirect proxies for enzymatic activity, such as posttranslational modifications to protein kinases. Our laboratories have recently described a method for directly quantifying the enzymatic activity of kinases in unfractionated cell lysates using substrates containing a phosphorylation-sensitive unnatural amino acid termed CSox, which can be monitored using fluorescence. Here, we demonstrate the utility of this method using a probe set encompassing p38α, MK2, ERK1/2, Akt, and PKA. This panel of chemosensors provides activity measurements of individual kinases in a model of skeletal muscle differentiation and can be readily used to generate individualized kinase activity profiles for tissue samples from clinical cancer patients.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Chemistry and Biology
Stains, Cliff I., Nathan C. Tedford, Traci C. Walkup, Elvedin Lukovic, Brenda N. Goguen, Linda G. Griffith, Douglas A. Lauffenburger, and Barbara Imperiali. “Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes.” Chemistry & Biology 19, no. 2 (February 2012): 210–217. © 2012 Elsevier Ltd.
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