Microfluidic jet injection for delivering macromolecules into cells

Author(s)
Roushdy, O.; Dokov, Rangel P.; Sharei, Armon Reza; Jensen, Klavs F.; Roushdy, O.; ... Show more
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Abstract
We present a microfluidic-based injection system designed to achieve intracellular delivery of macromolecules by directing a picoliter jet of a solution toward the individual cells. After discussing the concept, we present design specification and criteria, elucidate performance and discuss results. The method has the potential to be quantitative and of high throughput, overcoming the limitations of current intracellular delivery protocols.
Date issued
2013-02
URI
http://hdl.handle.net/1721.1/91556
Department
Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering; Koch Institute for Integrative Cancer Research at MIT
Journal
Journal of Micromechanics and Microengineering
Publisher
IOP Publishing
Citation
Adamo, A, O Roushdy, R Dokov, A Sharei, and K F Jensen. “Microfluidic Jet Injection for Delivering Macromolecules into Cells.” J. Micromech. Microeng. 23, no. 3 (February 6, 2013): 035026.
Version: Author's final manuscript
ISSN
0960-1317
1361-6439
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