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dc.contributor.authorGhosh, Debadyuti
dc.contributor.authorBelcher, Angela M.
dc.contributor.authorBardhan, Neelkanth Manoj
dc.date.accessioned2014-11-20T17:58:15Z
dc.date.available2014-11-20T17:58:15Z
dc.date.issued2013-04
dc.identifier.issn1864063X
dc.identifier.issn1864-0648
dc.identifier.urihttp://hdl.handle.net/1721.1/91654
dc.description.abstractWe report a first method for using M13 bacteriophage as a multifunctional scaffold for optically imaging bacterial infections in vivo. We demonstrate that M13 virus conjugated with hundreds of dye molecules (M13-Dye) can target and distinguish pathogenic infections of F- pili expressing and F -negative strains of E. coli. Further, in order to tune this M13-Dye complex suitable for targeting other strains of bacteria, we have used a 1-step reaction for creating an anti-bacterial antibody -M13-Dye probe. As an example, we show anti-S. aureus -M13-Dye able to target and image infections of S. aureus in living hosts, with a 3.7× increase in fluorescence over background.en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (Center for Cancer Nanotechnology Excellence Grant 5-U54-CA151884-03)en_US
dc.language.isoen_US
dc.publisherWiley Blackwellen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/jbio.201300010en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleM13 Virus based detection of bacterial infections in living hostsen_US
dc.typeArticleen_US
dc.identifier.citationBardhan, Neelkanth M., Debadyuti Ghosh, and Angela M. Belcher. “M13 Virus Based Detection of Bacterial Infections in Living Hosts.” Journal of Biophotonics 7, no. 8 (April 11, 2013): 617–623.en_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Materials Science and Engineeringen_US
dc.contributor.mitauthorBardhan, Neelkanth Manojen_US
dc.contributor.mitauthorGhosh, Debadyutien_US
dc.contributor.mitauthorBelcher, Angela M.en_US
dc.relation.journalJournal of Biophotonicsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsBardhan, Neelkanth M.; Ghosh, Debadyuti; Belcher, Angela M.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-4343-4007
dc.identifier.orcidhttps://orcid.org/0000-0001-9353-7453
mit.licenseOPEN_ACCESS_POLICYen_US


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