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dc.contributor.authorLuna, Rafael E.
dc.contributor.authorArthanari, Haribabu
dc.contributor.authorHiraishi, Hiroyuki
dc.contributor.authorNanda, Jagpreet
dc.contributor.authorMartin-Marcos, Pilar
dc.contributor.authorMarkus, Michelle A.
dc.contributor.authorAkabayov, Barak
dc.contributor.authorMilbradt, Alexander G.
dc.contributor.authorLuna, Lunet E.
dc.contributor.authorSeo, Hee-Chan
dc.contributor.authorHyberts, Sven G.
dc.contributor.authorFahmy, Amr
dc.contributor.authorReibarkh, Mikhail
dc.contributor.authorMiles, David
dc.contributor.authorHagner, Patrick R.
dc.contributor.authorO'Day, Elizabeth M.
dc.contributor.authorYi, Tingfang
dc.contributor.authorMarintchev, Assen
dc.contributor.authorHinnebusch, Alan G.
dc.contributor.authorLorsch, Jon R.
dc.contributor.authorAsano, Katsura
dc.contributor.authorWagner, Gerhard
dc.date.accessioned2014-11-24T15:40:49Z
dc.date.available2014-11-24T15:40:49Z
dc.date.issued2012-05
dc.date.submitted2012-02
dc.identifier.issn22111247
dc.identifier.urihttp://hdl.handle.net/1721.1/91690
dc.description.abstractRecognition of the proper start codon on mRNAs is essential for protein synthesis, which requires scanning and involves eukaryotic initiation factors (eIFs) eIF1, eIF1A, eIF2, and eIF5. The carboxyl terminal domain (CTD) of eIF5 stimulates 43S preinitiation complex (PIC) assembly; however, its precise role in scanning and start codon selection has remained unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we identified the binding sites of eIF1 and eIF2β on eIF5-CTD and found that they partially overlapped. Mutating select eIF5 residues in the common interface specifically disrupts interaction with both factors. Genetic and biochemical evidence indicates that these eIF5-CTD mutations impair start codon recognition and impede eIF1 release from the PIC by abrogating eIF5-CTD binding to eIF2β. This study provides mechanistic insight into the role of eIF5-CTD's dynamic interplay with eIF1 and eIF2β in switching PICs from an open to a closed state at start codons.en_US
dc.description.sponsorshipUnited States. Dept. of Energy. Office of Science (United States. Dept. of Energy. Office of Basic Energy Sciences. Contract DE-AC02-98CH10886)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.celrep.2012.04.007en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en_US
dc.sourceElsevieren_US
dc.titleThe C-Terminal Domain of Eukaryotic Initiation Factor 5 Promotes Start Codon Recognition by Its Dynamic Interplay with eIF1 and eIF2βen_US
dc.typeArticleen_US
dc.identifier.citationLuna, Rafael E., Haribabu Arthanari, Hiroyuki Hiraishi, Jagpreet Nanda, Pilar Martin-Marcos, Michelle A. Markus, Barak Akabayov, et al. “The C-Terminal Domain of Eukaryotic Initiation Factor 5 Promotes Start Codon Recognition by Its Dynamic Interplay with eIF1 and eIF2β.” Cell Reports 1, no. 6 (June 2012): 689–702.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorLuna, Lunet E.en_US
dc.relation.journalCell Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLuna, Rafael E.; Arthanari, Haribabu; Hiraishi, Hiroyuki; Nanda, Jagpreet; Martin-Marcos, Pilar; Markus, Michelle A.; Akabayov, Barak; Milbradt, Alexander G.; Luna, Lunet E.; Seo, Hee-Chan; Hyberts, Sven G.; Fahmy, Amr; Reibarkh, Mikhail; Miles, David; Hagner, Patrick R.; O'Day, Elizabeth M.; Yi, Tingfang; Marintchev, Assen; Hinnebusch, Alan G.; Lorsch, Jon R.; Asano, Katsura; Wagner, Gerharden_US
mit.licensePUBLISHER_CCen_US


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