dc.contributor.author | Khan, Mustafa | |
dc.contributor.author | Goldsmith, Christian R. | |
dc.contributor.author | Huang, Zhen | |
dc.contributor.author | Georgiou, John | |
dc.contributor.author | Luyben, Thomas T. | |
dc.contributor.author | Roder, John C. | |
dc.contributor.author | Lippard, Stephen J. | |
dc.contributor.author | Okamoto, Kenichi | |
dc.date.accessioned | 2014-12-01T18:32:32Z | |
dc.date.available | 2014-12-01T18:32:32Z | |
dc.date.issued | 2014-04 | |
dc.date.submitted | 2014-01 | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.issn | 1091-6490 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/91965 | |
dc.description.abstract | Mossy fiber termini in the hippocampus accumulate Zn[superscript 2+], which is released with glutamate from synaptic vesicles upon neural excitation. Understanding the spatiotemporal regulation of mobile Zn[superscript 2+] at the synaptic level is challenging owing to the difficulty of visualizing Zn[superscript 2+] at individual synapses. Here we describe the use of zinc-responsive fluorescent probes together with two-photon microscopy to image Zn[superscript 2+] dynamics mediated by NMDA receptor-dependent long-term potentiation induction at single mossy fiber termini of dentate gyrus neurons in adult mouse hippocampal slices. The membrane-impermeant fluorescent Zn[superscript 2+] probe, 6-CO2H-ZAP4, was loaded into presynaptic vesicles in hippocampal mossy fiber termini upon KCl-induced depolarization, which triggers subsequent endocytosis and vesicular restoration. Local tetanic stimulation decreased the Zn[superscript 2+] signal observed at individual presynaptic sites, indicating release of the Zn[superscript 2+] from vesicles in synaptic potentiation. This synapse-level two-photon Zn[superscript 2+] imaging method enables monitoring of presynaptic Zn[superscript 2+] dynamics for improving the understanding of physiological roles of mobile Zn[superscript 2+] in regular and aberrant neurologic function. | en_US |
dc.description.sponsorship | National Institute of General Medical Sciences (U.S.) (Grant GM 65519) | en_US |
dc.description.sponsorship | National Institute of Mental Health (U.S.) (Postdoctoral Fellowship) | en_US |
dc.language.iso | en_US | |
dc.publisher | National Academy of Sciences (U.S.) | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1073/pnas.1405154111 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | National Academy of Sciences (U.S.) | en_US |
dc.title | Two-photon imaging of Zn[superscript 2+] dynamics in mossy fiber boutons of adult hippocampal slices | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Khan, M., C. R. Goldsmith, Z. Huang, J. Georgiou, T. T. Luyben, J. C. Roder, S. J. Lippard, and K. Okamoto. “Two-Photon Imaging of Zn2+ Dynamics in Mossy Fiber Boutons of Adult Hippocampal Slices.” Proceedings of the National Academy of Sciences 111, no. 18 (April 22, 2014): 6786–6791. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Physics | en_US |
dc.contributor.mitauthor | Goldsmith, Christian R. | en_US |
dc.contributor.mitauthor | Huang, Zhen | en_US |
dc.contributor.mitauthor | Lippard, Stephen J. | en_US |
dc.relation.journal | Proceedings of the National Academy of Sciences of the United States of America | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Khan, Mustafa; Goldsmith, Christian R.; Huang, Zhen; Georgiou, John; Luyben, Thomas T.; Roder, John C.; Lippard, Stephen J.; Okamoto, Kenichi | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-2693-4982 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |