| dc.contributor.author | Rivera-Fuentes, Pablo | |
| dc.contributor.author | Lippard, Stephen J. | |
| dc.date.accessioned | 2015-02-24T15:21:06Z | |
| dc.date.available | 2015-02-24T15:21:06Z | |
| dc.date.issued | 2014-03 | |
| dc.date.submitted | 2014-01 | |
| dc.identifier.issn | 18607179 | |
| dc.identifier.issn | 1860-7187 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/95472 | |
| dc.description.abstract | A reversible, reaction-based sensor for biological mobile zinc was designed, prepared, and characterized. The sensing mechanism of this probe is based on the zinc-induced, ring-opening reaction of spirobenzopyran to give a cyanine fluorophore that emits in the deep-red region of the electromagnetic spectrum. This probe is not activated by protons and operates efficiently in aqueous solution at pH 7 and high ionic strength. The mechanism of this reaction was studied by using a combination of kinetics experiments and DFT calculations. The biocompatibility of the probe was demonstrated in live HeLa cells. | en_US |
| dc.description.sponsorship | National Institute of General Medical Sciences (U.S.) (Grant GM 65519) | en_US |
| dc.description.sponsorship | Swiss National Science Foundation (Postdoctoral Fellowship) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (1S0RR13886-01) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | Wiley Blackwell | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1002/cmdc.201400014 | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
| dc.source | PMC | en_US |
| dc.title | SpiroZin1: A Reversible and pH-Insensitive, Reaction-Based, Red-Fluorescent Probe for Imaging Biological Mobile Zinc | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Rivera-Fuentes, Pablo, and Stephen J. Lippard. “SpiroZin1: A Reversible and pH-Insensitive, Reaction-Based, Red-Fluorescent Probe for Imaging Biological Mobile Zinc.” ChemMedChem 9, no. 6 (March 11, 2014): 1238–1243. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
| dc.contributor.mitauthor | Lippard, Stephen J. | en_US |
| dc.contributor.mitauthor | Rivera-Fuentes, Pablo | en_US |
| dc.relation.journal | ChemMedChem | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Rivera-Fuentes, Pablo; Lippard, Stephen J. | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0002-2693-4982 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
| mit.metadata.status | Complete | |
