The class III ribonucleotide reductase from Neisseria bacilliformis can utilize thioredoxin as a reductant
Author(s)Wei, Yifeng; Funk, Michael Andrew; Rosado, Leonardo A.; Baek, Jiyeon; Drennan, Catherine L.; Stubbe, JoAnne; ... Show more Show less
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The class III anaerobic ribonucleotide reductases (RNRs) studied to date couple the reduction of ribonucleotides to deoxynucleotides with the oxidation of formate to CO[subscript 2]. Here we report the cloning and heterologous expression of the Neisseria bacilliformis class III RNR and show that it can catalyze nucleotide reduction using the ubiquitous thioredoxin/thioredoxin reductase/NADPH system. We present a structural model based on a crystal structure of the homologous Thermotoga maritima class III RNR, showing its architecture and the position of conserved residues in the active site. Phylogenetic studies suggest that this form of class III RNR is present in bacteria and archaea that carry out diverse types of anaerobic metabolism.
DepartmentMassachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Proceedings of the National Academy of Sciences of the United States of America
National Academy of Sciences (U.S.)
Wei, Y., M. A. Funk, L. A. Rosado, J. Baek, C. L. Drennan, and J. Stubbe. “The Class III Ribonucleotide Reductase from Neisseria Bacilliformis Can Utilize Thioredoxin as a Reductant.” Proceedings of the National Academy of Sciences 111, no. 36 (August 25, 2014): E3756–E3765.
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