High-Throughput Nonlinear Optical Microscopy
Author(s)Yew, Elijah Y.S.; So, Peter T. C.; Rowlands, Christopher; Yew, Yan Seng Elijah
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High-resolution microscopy methods based on different nonlinear optical (NLO) contrast mechanisms are finding numerous applications in biology and medicine. While the basic implementations of these microscopy methods are relatively mature, an important direction of continuing technological innovation lies in improving the throughput of these systems. Throughput improvement is expected to be important for studying fast kinetic processes, for enabling clinical diagnosis and treatment, and for extending the field of image informatics. This review will provide an overview of the fundamental limitations on NLO microscopy throughput. We will further cover several important classes of high-throughput NLO microscope designs with discussions on their strengths and weaknesses and their key biomedical applications. Finally, this review will close with a perspective of potential future technological improvements in this field.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Laser Biomedical Research Center
So, Peter T.C., Elijah Y.S. Yew, and Christopher Rowlands. “High-Throughput Nonlinear Optical Microscopy.” Biophysical Journal 105, no. 12 (December 2013): 2641–2654.
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