Solid-State NMR Characterization of Gas Vesicle Structure
Author(s)Sivertsen, Astrid C.; Bayro, Marvin J.; Belenky, Marina; Griffin, Robert Guy; Herzfeld, Judith
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Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-α-β-β-α-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a picture of the overall topology of the vesicle subunit that is consistent with its role in stabilizing an air-water interface.
DepartmentMassachusetts Institute of Technology. Department of Chemistry; Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
Sivertsen, Astrid C., Marvin J. Bayro, Marina Belenky, Robert G. Griffin, and Judith Herzfeld. “Solid-State NMR Characterization of Gas Vesicle Structure.” Biophysical Journal 99, no. 6 (September 2010): 1932–1939. © 2010 Biophysical Society.
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