Regulation of Spo12 Phosphorylation and Its Essential Role in the FEAR Network
Author(s)Tomson, Brett N.; Rahal, Rami; Reiser, Vladimír; Monje-Casas, Fernando; Mekhail, Karim; Moazed, Danesh; Amon, Angelika B.; ... Show more Show less
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Background: In budding yeast, the protein phosphatase Cdc14 coordinates late mitotic events and triggers exit from mitosis. During early anaphase, Cdc14 is activated by the FEAR network, but how signaling through the FEAR network occurs is poorly understood. Results: We find that the FEAR network component Spo12 is phosphorylated on S118. This phosphorylation is essential for Spo12 function and is restricted to early anaphase, when the FEAR network is active. The anaphase-specific phosphorylation of Spo12 requires mitotic CDKs and depends on the FEAR network components Separase and Slk19. Furthermore, we find that CDC14 is required to maintain Spo12 in the dephosphorylated state prior to anaphase. Conclusions: Our results show that anaphase-specific phosphorylation of Spo12 is essential for FEAR network function and raise the interesting possibility that Cdc14 itself helps to prevent the FEAR network from being prematurely activated.
DepartmentMassachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Tomson, Brett N., Rami Rahal, Vladimír Reiser, Fernando Monje-Casas, Karim Mekhail, Danesh Moazed, and Angelika Amon. “Regulation of Spo12 Phosphorylation and Its Essential Role in the FEAR Network.” Current Biology 19, no. 6 (March 2009): 449–460. © 2009 Elsevier Ltd.
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