| dc.contributor.author | Wrobel, Alexandra T. | |
| dc.contributor.author | Lippard, Stephen J. | |
| dc.contributor.author | Rivera-Fuentes, Pablo | |
| dc.contributor.author | Johnstone, Timothy | |
| dc.contributor.author | Liang, Alexandria D. | |
| dc.date.accessioned | 2015-03-30T17:14:02Z | |
| dc.date.available | 2015-03-30T17:14:02Z | |
| dc.date.issued | 2014-02 | |
| dc.date.submitted | 2014-01 | |
| dc.identifier.issn | 0002-7863 | |
| dc.identifier.issn | 1520-5126 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/96249 | |
| dc.description.abstract | The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live mammalian cells and in conjunction with the zinc-specific, green-fluorescent sensor ZP1 can perform multicolor/multianalyte microscopic imaging. These studies reveal that HNO treatment elicits an increase in the concentration of intracellular mobile zinc. | en_US |
| dc.description.sponsorship | National Science Foundation (U.S.) (Grant CHE-1265770) | en_US |
| dc.description.sponsorship | Massachusetts Institute of Technology. Undergraduate Research Opportunities Program Paul E. Gray (1954) Endowed Fund) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (Interdepartmental Biotechnology Training Grant T32 GM008334) | en_US |
| dc.description.sponsorship | Swiss National Science Foundation (Postdoctoral Fellowship) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | American Chemical Society (ACS) | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1021/ja500315x | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
| dc.source | Prof. Lippard via Erja Kajosalo | en_US |
| dc.title | A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO) | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Wrobel, Alexandra T., Timothy C. Johnstone, Alexandria Deliz Liang, Stephen J. Lippard, and Pablo Rivera-Fuentes. “A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO).” Journal of the American Chemical Society 136, no. 12 (March 26, 2014): 4697–4705. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
| dc.contributor.mitauthor | Lippard, Stephen J. | en_US |
| dc.contributor.mitauthor | Wrobel, Alexandra T. | en_US |
| dc.contributor.mitauthor | Johnstone, Timothy | en_US |
| dc.contributor.mitauthor | Liang, Alexandria D. | en_US |
| dc.contributor.mitauthor | Rivera-Fuentes, Pablo | en_US |
| dc.relation.journal | Journal of the American Chemical Society | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Wrobel, Alexandra T.; Johnstone, Timothy C.; Deliz Liang, Alexandria; Lippard, Stephen J.; Rivera-Fuentes, Pablo | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0002-2693-4982 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
| mit.metadata.status | Complete | |
