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dc.contributor.authorSoldner, Frank
dc.contributor.authorHockemeyer, Dirk
dc.contributor.authorBeard, Caroline
dc.contributor.authorGao, Qing
dc.contributor.authorBell, George W.
dc.contributor.authorCook, Elizabeth G.
dc.contributor.authorHargus, Gunnar
dc.contributor.authorBlak, Alexandra
dc.contributor.authorCooper, Oliver
dc.contributor.authorMitalipova, Maisam
dc.contributor.authorIsacson, Ole
dc.contributor.authorJaenisch, Rudolf
dc.date.accessioned2015-03-30T20:35:48Z
dc.date.available2015-03-30T20:35:48Z
dc.date.issued2009-03
dc.date.submitted2009-01
dc.identifier.issn00928674
dc.identifier.urihttp://hdl.handle.net/1721.1/96268
dc.description.abstractInduced pluripotent stem cells (iPSCs) derived from somatic cells of patients represent a powerful tool for biomedical research and may provide a source for replacement therapies. However, the use of viruses encoding the reprogramming factors represents a major limitation of the current technology since even low vector expression may alter the differentiation potential of the iPSCs or induce malignant transformation. Here, we show that fibroblasts from five patients with idiopathic Parkinson's disease can be efficiently reprogrammed and subsequently differentiated into dopaminergic neurons. Moreover, we derived hiPSCs free of reprogramming factors using Cre-recombinase excisable viruses. Factor-free hiPSCs maintain a pluripotent state and show a global gene expression profile, more closely related to hESCs than to hiPSCs carrying the transgenes. Our results indicate that residual transgene expression in virus-carrying hiPSCs can affect their molecular characteristics and that factor-free hiPSCs therefore represent a more suitable source of cells for modeling of human disease.en_US
dc.description.sponsorshipHoward Hughes Medical Institute (Collaborative Innovation Award)en_US
dc.description.sponsorshipLife Sciences Research Foundation (Merck Fellow)en_US
dc.description.sponsorshipMichael Stern Parkinson's Research Foundationen_US
dc.description.sponsorshipMorris K. Udall Center for Excellence in Parkinson’s Research (grant P50NS39793)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH grant R37-CA084198)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH grant RO1-CA087869)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (grant NIH RO1-HD045022)en_US
dc.language.isoen_US
dc.publisherElsevier B.V.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.cell.2009.02.013en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceElsevieren_US
dc.titleParkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factorsen_US
dc.typeArticleen_US
dc.identifier.citationSoldner, Frank, Dirk Hockemeyer, Caroline Beard, Qing Gao, George W. Bell, Elizabeth G. Cook, Gunnar Hargus, et al. “Parkinson’s Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors.” Cell 136, no. 5 (March 2009): 964–977. © 2009 Elsevier Inc.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorSoldner, Franken_US
dc.contributor.mitauthorHockemeyer, Dirken_US
dc.contributor.mitauthorBeard, Carolineen_US
dc.contributor.mitauthorGao, Qingen_US
dc.contributor.mitauthorBell, George W.en_US
dc.contributor.mitauthorCook, Elizabeth G.en_US
dc.contributor.mitauthorMitalipova, Maisamen_US
dc.contributor.mitauthorJaenisch, Rudolfen_US
dc.relation.journalCellen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSoldner, Frank; Hockemeyer, Dirk; Beard, Caroline; Gao, Qing; Bell, George W.; Cook, Elizabeth G.; Hargus, Gunnar; Blak, Alexandra; Cooper, Oliver; Mitalipova, Maisam; Isacson, Ole; Jaenisch, Rudolfen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-9724-6776
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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