dc.contributor.author | Kiyomitsu, Tomomi | |
dc.contributor.author | Cheeseman, Iain M | |
dc.date.accessioned | 2015-03-31T16:56:14Z | |
dc.date.available | 2015-03-31T16:56:14Z | |
dc.date.issued | 2013-07 | |
dc.date.submitted | 2013-04 | |
dc.identifier.issn | 00928674 | |
dc.identifier.issn | 1097-4172 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/96283 | |
dc.description.abstract | Mitotic spindle position defines the cell-cleavage site during cytokinesis. However, the mechanisms that control spindle positioning to generate equal-sized daughter cells remain poorly understood. Here, we demonstrate that two mechanisms act coordinately to center the spindle during anaphase in symmetrically dividing human cells. First, the spindle is positioned directly by the microtubule-based motor dynein, which we demonstrate is targeted to the cell cortex by two distinct pathways: a Gαi/LGN/NuMA-dependent pathway and a 4.1G/R and NuMA-dependent, anaphase-specific pathway. Second, we find that asymmetric plasma membrane elongation occurs in response to spindle mispositioning to alter the cellular boundaries relative to the spindle. Asymmetric membrane elongation is promoted by chromosome-derived Ran-GTP signals that locally reduce Anillin at the growing cell cortex. In asymmetrically elongating cells, dynein-dependent spindle anchoring at the stationary cell cortex ensures proper spindle positioning. Our results reveal the anaphase-specific spindle centering systems that achieve equal-sized cell division. | en_US |
dc.description.sponsorship | Leukemia & Lymphoma Society of America (Scholar Award) | en_US |
dc.description.sponsorship | National Institute of General Medical Sciences (U.S.) (GM088313) | en_US |
dc.description.sponsorship | American Cancer Society (Research Scholar Grant 121776) | en_US |
dc.description.sponsorship | Human Frontier Science Program (Strasbourg, France) (Award) | en_US |
dc.description.sponsorship | Human Frontier Science Program (Strasbourg, France) (Long-Term Fellowship) | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/j.cell.2013.06.010 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | Elsevier Open Archive | en_US |
dc.title | Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Kiyomitsu, Tomomi, and Iain M. Cheeseman. “Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase.” Cell 154, no. 2 (July 2013): 391–402. © 2013 Elsevier Inc. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Cheeseman, Iain McPherson | en_US |
dc.contributor.mitauthor | Kiyomitsu, Tomomi | en_US |
dc.relation.journal | Cell | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Kiyomitsu, Tomomi; Cheeseman, Iain M. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3829-5612 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |