TALEN-mediated editing of the mouse Y chromosome
Author(s)Wang, Haoyi; Hu, Yueh-Chiang; Markoulaki, Styliani; Welstead, G Grant; Cheng, Albert W.; Shivalila, Chikdu Shakti; Pyntikova, Tatyana; Dadon, Daniel Benjamin; Voytas, Daniel F.; Bogdanove, Adam J.; Page, David C.; Jaenisch, Rudolf; Welstead, G. Grant; ... Show more Show less
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The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes—Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.
DepartmentMassachusetts Institute of Technology. Computational and Systems Biology Program; Massachusetts Institute of Technology. Department of Biology; Whitehead Institute for Biomedical Research
Nature Publishing Group
Wang, Haoyi, Yueh-Chiang Hu, Styliani Markoulaki, G Grant Welstead, Albert W Cheng, Chikdu S Shivalila, Tatyana Pyntikova, et al. “TALEN-Mediated Editing of the Mouse Y Chromosome.” Nature Biotechnology 31, no. 6 (May 12, 2013): 530–532.
Author's final manuscript