Topological organization of multi-chromosomal regions by Firre
Author(s)Hacisuleyman, Ezgi; Trapnell, Cole; Williams, Adam; Henao-Mejia, Jorge; Sun, Lei; McClanahan, Patrick; Hendrickson, David G.; Sauvageau, Martin; Kelley, David R.; Morse, Michael; Engreitz, Jesse Michael; Guttman, Mitchell; Flavell, Richard A.; Raj, Arjun; Rinn, John L.; Goff, Loyal; Lander, Eric S.; Lodish, Harvey F.; ... Show more Show less
Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre
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RNA, including long noncoding RNA (lncRNA), is known to be an abundant and important structural component of the nuclear matrix. However, the molecular identities, functional roles and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we describe one lncRNA, Firre, that interacts with the nuclear-matrix factor hnRNPU through a 156-bp repeating sequence and localizes across an ~5-Mb domain on the X chromosome. We further observed Firre localization across five distinct trans-chromosomal loci, which reside in spatial proximity to the Firre genomic locus on the X chromosome. Both genetic deletion of the Firre locus and knockdown of hnRNPU resulted in loss of colocalization of these trans-chromosomal interacting loci. Thus, our data suggest a model in which lncRNAs such as Firre can interface with and modulate nuclear architecture across chromosomes.
DepartmentMassachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology
Nature Structural & Molecular Biology
Nature Publishing Group
Hacisuleyman, Ezgi, Loyal A Goff, Cole Trapnell, Adam Williams, Jorge Henao-Mejia, Lei Sun, Patrick McClanahan, et al. “Topological Organization of Multichromosomal Regions by the Long Intergenic Noncoding RNA Firre.” Nature Structural & Molecular Biology 21, no. 2 (January 26, 2014): 198–206.
Author's final manuscript