Fully-automated in vivo single cell electrophysiology
Author(s)Go, Jamison; Fan, Aaron; Lu, Coby; Kodandaramaiah, Suhasa Bangalo; Holst, Gregory L.; Stoy, William; Kolb, Ilya; Boyden, Edward Stuart; Forest, Craig R.; ... Show more Show less
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In this work, we report progress in developing a device that allows fully autonomous sequential patch clamp experimentation. The machine works by integrating a storage magazine of pre-filled pipettes that can be accessed, and swapped, by the headstage at the conclusion of each experiment. In operation, following each neuron measurement, the program enters “swap” state where a set of programmed actuator movements take place. First, the headstage translates towards the pipette storage assembly and deposits its used pipette. The storage assembly rotates to index a fresh pipette, its is grasped, and finally, the headstage returns to its previously designated home position in preparation of subsequent experiments.
DepartmentMassachusetts Institute of Technology. Media Laboratory; McGovern Institute for Brain Research at MIT; Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Proceedings of the 28th Annual Meeting of the American Society for Precision Engineering
American Society for Precision Engineering
Go, Jamison, Aaron Fan, Coby Lu, Suhasa Kodandaramaiah, Gregory L. Holst, William Stoy, Ilya Kolb, Edward S. Boyden, and Craig R. Forest (2013). "Fully-automated, in-vivo, single cell electrophysiology" in Proceedings of the 28th Annual Meeting of the American Society for Precision Engineering, Saint Paul, MN, Oct 20-25, 2013.
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