dc.contributor.author | Hu, Yueh-Chiang | |
dc.contributor.author | Nicholls, Peter K. | |
dc.contributor.author | Soh, Ying Qi Shirleen | |
dc.contributor.author | Daniele, Joseph R. | |
dc.contributor.author | Junker, Jan Philipp | |
dc.contributor.author | van Oudenaarden, Alexander | |
dc.contributor.author | Page, David C | |
dc.date.accessioned | 2015-04-23T17:28:17Z | |
dc.date.available | 2015-04-23T17:28:17Z | |
dc.date.issued | 2015-03 | |
dc.date.submitted | 2014-08 | |
dc.identifier.issn | 1553-7404 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/96744 | |
dc.description.abstract | In mouse embryos at mid-gestation, primordial germ cells (PGCs) undergo licensing to become gametogenesis-competent cells (GCCs), gaining the capacity for meiotic initiation and sexual differentiation. GCCs then initiate either oogenesis or spermatogenesis in response to gonadal cues. Germ cell licensing has been considered to be a cell-autonomous and gonad-independent event, based on observations that some PGCs, having migrated not to the gonad but to the adrenal gland, nonetheless enter meiosis in a time frame parallel to ovarian germ cells -- and do so regardless of the sex of the embryo. Here we test the hypothesis that germ cell licensing is cell-autonomous by examining the fate of PGCs in Gata4 conditional mutant (Gata4 cKO) mouse embryos. Gata4, which is expressed only in somatic cells, is known to be required for genital ridge initiation. PGCs in Gata4 cKO mutants migrated to the area where the genital ridge, the precursor of the gonad, would ordinarily be formed. However, these germ cells did not undergo licensing and instead retained characteristics of PGCs. Our results indicate that licensing is not purely cell-autonomous but is induced by the somatic genital ridge. | en_US |
dc.description.sponsorship | National Health and Medical Research Council (Australia) (CJ Martin Fellowship, #1053776) | en_US |
dc.description.sponsorship | Howard Hughes Medical Institute (Investigator) | en_US |
dc.language.iso | en_US | |
dc.publisher | Public Library of Science | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1371/journal.pgen.1005019 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Public Library of Science | en_US |
dc.title | Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Hu, Yueh-Chiang, Peter K. Nicholls, Y. Q. Shirleen Soh, Joseph R. Daniele, Jan Philipp Junker, Alexander van Oudenaarden, and David C. Page. “Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling.” Edited by R. Scott Hawley. PLoS Genet 11, no. 3 (March 4, 2015): e1005019. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Physics | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Hu, Yueh-Chiang | en_US |
dc.contributor.mitauthor | Nicholls, Peter K. | en_US |
dc.contributor.mitauthor | Soh, Ying Qi Shirleen | en_US |
dc.contributor.mitauthor | Daniele, Joseph R. | en_US |
dc.contributor.mitauthor | van Oudenaarden, Alexander | en_US |
dc.contributor.mitauthor | Page, David C. | en_US |
dc.relation.journal | PLOS Genetics | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Hu, Yueh-Chiang; Nicholls, Peter K.; Soh, Y. Q. Shirleen; Daniele, Joseph R.; Junker, Jan Philipp; van Oudenaarden, Alexander; Page, David C. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-9920-3411 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |