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dc.contributor.authorHu, Yueh-Chiang
dc.contributor.authorNicholls, Peter K.
dc.contributor.authorSoh, Ying Qi Shirleen
dc.contributor.authorDaniele, Joseph R.
dc.contributor.authorJunker, Jan Philipp
dc.contributor.authorvan Oudenaarden, Alexander
dc.contributor.authorPage, David C
dc.date.accessioned2015-04-23T17:28:17Z
dc.date.available2015-04-23T17:28:17Z
dc.date.issued2015-03
dc.date.submitted2014-08
dc.identifier.issn1553-7404
dc.identifier.urihttp://hdl.handle.net/1721.1/96744
dc.description.abstractIn mouse embryos at mid-gestation, primordial germ cells (PGCs) undergo licensing to become gametogenesis-competent cells (GCCs), gaining the capacity for meiotic initiation and sexual differentiation. GCCs then initiate either oogenesis or spermatogenesis in response to gonadal cues. Germ cell licensing has been considered to be a cell-autonomous and gonad-independent event, based on observations that some PGCs, having migrated not to the gonad but to the adrenal gland, nonetheless enter meiosis in a time frame parallel to ovarian germ cells -- and do so regardless of the sex of the embryo. Here we test the hypothesis that germ cell licensing is cell-autonomous by examining the fate of PGCs in Gata4 conditional mutant (Gata4 cKO) mouse embryos. Gata4, which is expressed only in somatic cells, is known to be required for genital ridge initiation. PGCs in Gata4 cKO mutants migrated to the area where the genital ridge, the precursor of the gonad, would ordinarily be formed. However, these germ cells did not undergo licensing and instead retained characteristics of PGCs. Our results indicate that licensing is not purely cell-autonomous but is induced by the somatic genital ridge.en_US
dc.description.sponsorshipNational Health and Medical Research Council (Australia) (CJ Martin Fellowship, #1053776)en_US
dc.description.sponsorshipHoward Hughes Medical Institute (Investigator)en_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pgen.1005019en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourcePublic Library of Scienceen_US
dc.titleLicensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signalingen_US
dc.typeArticleen_US
dc.identifier.citationHu, Yueh-Chiang, Peter K. Nicholls, Y. Q. Shirleen Soh, Joseph R. Daniele, Jan Philipp Junker, Alexander van Oudenaarden, and David C. Page. “Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling.” Edited by R. Scott Hawley. PLoS Genet 11, no. 3 (March 4, 2015): e1005019.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Physicsen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorHu, Yueh-Chiangen_US
dc.contributor.mitauthorNicholls, Peter K.en_US
dc.contributor.mitauthorSoh, Ying Qi Shirleenen_US
dc.contributor.mitauthorDaniele, Joseph R.en_US
dc.contributor.mitauthorvan Oudenaarden, Alexanderen_US
dc.contributor.mitauthorPage, David C.en_US
dc.relation.journalPLOS Geneticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHu, Yueh-Chiang; Nicholls, Peter K.; Soh, Y. Q. Shirleen; Daniele, Joseph R.; Junker, Jan Philipp; van Oudenaarden, Alexander; Page, David C.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-9920-3411
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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