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Single Cell Confocal Raman Spectroscopy of Human Osteoarthritic Chondrocytes: A Preliminary Study

Author(s)
Kumar, Rajesh; Afseth, Nils; de Lange Davies, Catharina; Drogset, Jon; Lilledahl, Magnus; Singh, Gajendra Pratap; Gronhaug, Kirsten; ... Show more Show less
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Abstract
A great deal of effort has been focused on exploring the underlying molecular mechanism of osteoarthritis (OA) especially at the cellular level. We report a confocal Raman spectroscopic investigation on human osteoarthritic chondrocytes. The objective of this investigation is to identify molecular features and the stage of OA based on the spectral signatures corresponding to bio-molecular changes at the cellular level in chondrocytes. In this study, we isolated chondrocytes from human osteoarthritic cartilage and acquired Raman spectra from single cells. Major spectral differences between the cells obtained from different International Cartilage Repair Society (ICRS) grades of osteoarthritic cartilage were identified. During progression of OA, a decrease in protein content and an increase in cell death were observed from the vibrational spectra. Principal component analysis and subsequent cross-validation was able to associate osteoarthritic chondrocytes to ICRS Grade I, II and III with specificity 100.0%, 98.1%, and 90.7% respectively, while, sensitivity was 98.6%, 82.8%, and 97.5% respectively. The overall predictive efficiency was 92.2%. Our pilot study encourages further use of Raman spectroscopy as a noninvasive and label free technique for revealing molecular features associated with osteoarthritic chondrocytes.
Date issued
2015-04
URI
http://hdl.handle.net/1721.1/97098
Department
Massachusetts Institute of Technology. Research Laboratory of Electronics
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Citation
Kumar, Rajesh, Gajendra Singh, Kirsten Gronhaug, Nils Afseth, Catharina de Lange Davies, Jon Drogset, and Magnus Lilledahl. “Single Cell Confocal Raman Spectroscopy of Human Osteoarthritic Chondrocytes: A Preliminary Study.” International Journal of Molecular Sciences 16, no. 5 (April 24, 2015): 9341–9353.
Version: Final published version
ISSN
1422-0067

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