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dc.contributor.authorSinha, Ameya
dc.contributor.authorChu, Trang T. T.
dc.contributor.authorDao, Ming
dc.contributor.authorChandramohanadas, Rajesh
dc.date.accessioned2015-06-09T18:00:10Z
dc.date.available2015-06-09T18:00:10Z
dc.date.issued2015-05
dc.date.submitted2014-11
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/97250
dc.description.abstractErythroid cells, specifically red blood cells (RBCs), are constantly exposed to highly reactive radicals during cellular gaseous exchange. Such exposure often exceeds the cells' innate anti-oxidant defense systems, leading to progressive damage and eventual senescence. One of the contributing factors to this process are alterations to hemoglobin conformation and globin binding to red cell cytoskeleton. However, in addition to the aforementioned changes, it is possible that oxidative damage induces critical changes to the erythrocyte cytoskeleton and corresponding bio-mechanical and nano-structural properties of the red cell membrane. To quantitatively characterize how oxidative damage accounts for such changes, we employed single-cell manipulation techniques such as micropipette aspiration and atomic force microscopy (AFM) on RBCs. These investigations demonstrated visible morphological changes upon chemically induced oxidative damage (using hydrogen peroxide, diamide, primaquine bisphosphate and cumene hydroperoxide). Our results provide previously unavailable observations on remarkable changes in red cell cytoskeletal architecture and membrane stiffness due to oxidative damage. Furthermore, we also demonstrate that a pathogen that infects human blood cells, Plasmodium falciparum was unable to penetrate through the oxidant-exposed RBCs that have damaged cytoskeleton and stiffer membranes. This indicates the importance of bio-physical factors pertinent to aged RBCs and it's relevance to malaria infectivity.en_US
dc.description.sponsorshipSingapore-MIT Alliance for Research and Technology (Singapore. National Research Foundation)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep09768en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleSingle-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stressen_US
dc.typeArticleen_US
dc.identifier.citationSinha, Ameya, Trang T. T. Chu, Ming Dao, and Rajesh Chandramohanadas. “Single-Cell Evaluation of Red Blood Cell Bio-Mechanical and Nano-Structural Alterations Upon Chemically Induced Oxidative Stress.” Sci. Rep. 5 (May 7, 2015): 9768.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Materials Science and Engineeringen_US
dc.contributor.mitauthorDao, Mingen_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSinha, Ameya; Chu, Trang T. T.; Dao, Ming; Chandramohanadas, Rajeshen_US
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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