dc.contributor.author | Li, Yingxiang | |
dc.contributor.author | Mou, Haiwei | |
dc.contributor.author | Colpan, Cansu | |
dc.contributor.author | Bizhanova, Aizhan | |
dc.contributor.author | Akama-Garren, Elliot | |
dc.contributor.author | Joshi, Nik | |
dc.contributor.author | Feldser, David M. | |
dc.contributor.author | Yin, Hao | |
dc.contributor.author | Weng, Zhiping | |
dc.contributor.author | Xue, Wen | |
dc.contributor.author | Park, Angela I. | |
dc.contributor.author | Hendrickson, Eric A. | |
dc.contributor.author | Anderson, Daniel Griffith | |
dc.contributor.author | Jacks, Tyler E | |
dc.date.accessioned | 2015-06-29T18:06:07Z | |
dc.date.available | 2015-06-29T18:06:07Z | |
dc.date.issued | 2015-05 | |
dc.date.submitted | 2015-03 | |
dc.identifier.issn | 1465-6906 | |
dc.identifier.issn | 1474-7596 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/97565 | |
dc.description.abstract | Although chromosomal deletions and inversions are important in cancer, conventional methods for detecting DNA rearrangements require laborious indirect assays. Here we develop fluorescent reporters to rapidly quantify CRISPR/Cas9-mediated deletions and inversions. We find that inversion depends on the non-homologous end-joining enzyme LIG4. We also engineer deletions and inversions for a 50 kb Pten genomic region in mouse liver. We discover diverse yet sequence-specific indels at the rearrangement fusion sites. Moreover, we detect Cas9 cleavage at the fourth nucleotide on the non-complementary strand, leading to staggered instead of blunt DNA breaks. These reporters allow mechanisms of chromosomal rearrangements to be investigated. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 2-PO1-CA42063) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant RO1-EB000244) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant RO1-CA115527) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant RO1-CA132091) | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (Cancer Center Support (Core) Grant P30-CA14051) | en_US |
dc.description.sponsorship | MIT Skoltech Initiative | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Centers for Cancer Nanotechnology Excellence 5-U54-CA151884-04) | en_US |
dc.description.sponsorship | MIT-Harvard Center of Cancer Nanotechnology Excellence | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1186/s13059-015-0680-7 | en_US |
dc.title | A versatile reporter system for CRISPR-mediated chromosomal rearrangements | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Li, Yingxiang, Angela I. Park, Haiwei Mou, Cansu Colpan, Aizhan Bizhanova, Elliot Akama-Garren, Nik Joshi, et al. “A Versatile Reporter System for CRISPR-Mediated Chromosomal Rearrangements.” Genome Biology 16, no. 1 (May 28, 2015). | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Institute for Medical Engineering & Science | en_US |
dc.contributor.department | Harvard University--MIT Division of Health Sciences and Technology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.mitauthor | Akama-Garren, Elliot | en_US |
dc.contributor.mitauthor | Joshi, Nik | en_US |
dc.contributor.mitauthor | Yin, Hao | en_US |
dc.contributor.mitauthor | Anderson, Daniel Griffith | en_US |
dc.contributor.mitauthor | Jacks, Tyler E. | en_US |
dc.relation.journal | Genome Biology | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2015-06-29T08:40:52Z | |
dc.language.rfc3066 | en | |
dc.rights.holder | Li et al. | |
dspace.orderedauthors | Li, Yingxiang; Park, Angela I.; Mou, Haiwei; Colpan, Cansu; Bizhanova, Aizhan; Akama-Garren, Elliot; Joshi, Nik; Hendrickson, Eric A.; Feldser, David; Yin, Hao; Anderson, Daniel G.; Jacks, Tyler; Weng, Zhiping; Xue, Wen | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-5785-8911 | |
dc.identifier.orcid | https://orcid.org/0000-0002-7045-7837 | |
dc.identifier.orcid | https://orcid.org/0000-0001-5629-4798 | |
dc.identifier.orcid | https://orcid.org/0000-0001-6898-3793 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |