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dc.contributor.authorLi, Yingxiang
dc.contributor.authorMou, Haiwei
dc.contributor.authorColpan, Cansu
dc.contributor.authorBizhanova, Aizhan
dc.contributor.authorAkama-Garren, Elliot
dc.contributor.authorJoshi, Nik
dc.contributor.authorFeldser, David M.
dc.contributor.authorYin, Hao
dc.contributor.authorWeng, Zhiping
dc.contributor.authorXue, Wen
dc.contributor.authorPark, Angela I.
dc.contributor.authorHendrickson, Eric A.
dc.contributor.authorAnderson, Daniel Griffith
dc.contributor.authorJacks, Tyler E
dc.date.accessioned2015-06-29T18:06:07Z
dc.date.available2015-06-29T18:06:07Z
dc.date.issued2015-05
dc.date.submitted2015-03
dc.identifier.issn1465-6906
dc.identifier.issn1474-7596
dc.identifier.urihttp://hdl.handle.net/1721.1/97565
dc.description.abstractAlthough chromosomal deletions and inversions are important in cancer, conventional methods for detecting DNA rearrangements require laborious indirect assays. Here we develop fluorescent reporters to rapidly quantify CRISPR/Cas9-mediated deletions and inversions. We find that inversion depends on the non-homologous end-joining enzyme LIG4. We also engineer deletions and inversions for a 50 kb Pten genomic region in mouse liver. We discover diverse yet sequence-specific indels at the rearrangement fusion sites. Moreover, we detect Cas9 cleavage at the fourth nucleotide on the non-complementary strand, leading to staggered instead of blunt DNA breaks. These reporters allow mechanisms of chromosomal rearrangements to be investigated.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 2-PO1-CA42063)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant RO1-EB000244)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant RO1-CA115527)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant RO1-CA132091)en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (Cancer Center Support (Core) Grant P30-CA14051)en_US
dc.description.sponsorshipMIT Skoltech Initiativeen_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Centers for Cancer Nanotechnology Excellence 5-U54-CA151884-04)en_US
dc.description.sponsorshipMIT-Harvard Center of Cancer Nanotechnology Excellenceen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/s13059-015-0680-7en_US
dc.titleA versatile reporter system for CRISPR-mediated chromosomal rearrangementsen_US
dc.typeArticleen_US
dc.identifier.citationLi, Yingxiang, Angela I. Park, Haiwei Mou, Cansu Colpan, Aizhan Bizhanova, Elliot Akama-Garren, Nik Joshi, et al. “A Versatile Reporter System for CRISPR-Mediated Chromosomal Rearrangements.” Genome Biology 16, no. 1 (May 28, 2015).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorAkama-Garren, Ellioten_US
dc.contributor.mitauthorJoshi, Niken_US
dc.contributor.mitauthorYin, Haoen_US
dc.contributor.mitauthorAnderson, Daniel Griffithen_US
dc.contributor.mitauthorJacks, Tyler E.en_US
dc.relation.journalGenome Biologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2015-06-29T08:40:52Z
dc.language.rfc3066en
dc.rights.holderLi et al.
dspace.orderedauthorsLi, Yingxiang; Park, Angela I.; Mou, Haiwei; Colpan, Cansu; Bizhanova, Aizhan; Akama-Garren, Elliot; Joshi, Nik; Hendrickson, Eric A.; Feldser, David; Yin, Hao; Anderson, Daniel G.; Jacks, Tyler; Weng, Zhiping; Xue, Wenen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-5785-8911
dc.identifier.orcidhttps://orcid.org/0000-0002-7045-7837
dc.identifier.orcidhttps://orcid.org/0000-0001-5629-4798
dc.identifier.orcidhttps://orcid.org/0000-0001-6898-3793
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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