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dc.contributor.authorCha, Jae Won
dc.contributor.authorSubramanian, Jaichandar
dc.contributor.authorNedivi, Elly
dc.contributor.authorSo, Peter T. C.
dc.contributor.authorYew, Yan Seng Elijah
dc.contributor.authorKim, Daekeun, Ph. D. Massachusetts Institute of Technology
dc.date.accessioned2015-07-13T14:56:56Z
dc.date.available2015-07-13T14:56:56Z
dc.date.issued2015-03
dc.date.submitted2014-09
dc.identifier.issn2158-3226
dc.identifier.urihttp://hdl.handle.net/1721.1/97725
dc.description.abstractMultifocal multiphoton microscopy (MMM) improves imaging speed over a point scanning approach by parallelizing the excitation process. Early versions of MMM relied on imaging detectors to record emission signals from multiple foci simultaneously. For many turbid biological specimens, the scattering of emission photons results in blurred images and degrades the signal-to-noise ratio (SNR). We have recently demonstrated that a multianode photomultiplier tube (MAPMT) placed in a descanned configuration can effectively collect scattered emission photons from each focus into their corresponding anodes significantly improving image SNR for highly scattering specimens. Unfortunately, a descanned MMM has a longer detection path resulting in substantial emission photon loss. Optical design constraints in a descanned geometry further results in significant optical aberrations especially for large field-of-view (FOV), high NA objectives. Here, we introduce a non-descanned MMM based on MAPMT that substantially overcomes most of these drawbacks. We show that we improve signal efficiency up to fourfold with limited image SNR degradation due to scattered emission photons. The excitation foci can also be spaced wider to cover the full FOV of the objective with minimal aberrations. The performance of this system is demonstrated by imaging interneuron morphological structures deep in the brains of living mice.en_US
dc.description.sponsorshipGrant RO1 EY017656en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (9P41EB015871)en_US
dc.description.sponsorship5 R01 NS051320en_US
dc.description.sponsorship4R44EB012415en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (CBET-0939511)en_US
dc.description.sponsorshipSingapore-MIT Alliance for Research and Technologyen_US
dc.description.sponsorshipMIT Skoltech Initiativeen_US
dc.description.sponsorshipHamamatsu Corporationen_US
dc.description.sponsorshipDavid H. Koch Institute for Integrative Cancer Research at MIT (Bridge Project Initiative)en_US
dc.language.isoen_US
dc.publisherAmerican Institute of Physics (AIP)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1063/1.4916040en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleNon-descanned multifocal multiphoton microscopy with a multianode photomultiplier tubeen_US
dc.typeArticleen_US
dc.identifier.citationCha, Jae Won, Elijah Y. S. Yew, Daekeun Kim, Jaichandar Subramanian, Elly Nedivi, and Peter T. C. So. “Non-Descanned Multifocal Multiphoton Microscopy with a Multianode Photomultiplier Tube.” AIP Advances 5, no. 8 (August 2015): 084802.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.departmentPicower Institute for Learning and Memoryen_US
dc.contributor.mitauthorCha, Jae Wonen_US
dc.contributor.mitauthorYew, Elijah Y. S.en_US
dc.contributor.mitauthorSubramanian, Jaichandaren_US
dc.contributor.mitauthorNedivi, Ellyen_US
dc.contributor.mitauthorSo, Peter T. C.en_US
dc.relation.journalAIP Advancesen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsCha, Jae Won; Yew, Elijah Y. S.; Kim, Daekeun; Subramanian, Jaichandar; Nedivi, Elly; So, Peter T. C.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-1710-0767
dc.identifier.orcidhttps://orcid.org/0000-0003-4698-6488
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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