Three-dimensional image cytometer based on widefield structured light microscopy and high-speed remote depth scanning
Author(s)Choi, Heejin; Wadduwage, Dushan N.; Tu, Ting Yuan; Matsudaira, Paul T.; So, Peter T. C.
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A high throughput 3D image cytometer have been developed that improves imaging speed by an order of magnitude over current technologies. This imaging speed improvement was realized by combining several key components. First, a depth-resolved image can be rapidly generated using a structured light reconstruction algorithm that requires only two wide field images, one with uniform illumination and the other with structured illumination. Second, depth scanning is implemented using the high speed remote depth scanning. Finally, the large field of view, high NA objective lens and the high pixelation, high frame rate sCMOS camera enable high resolution, high sensitivity imaging of a large cell population. This system can image at 800 cell/sec in 3D at submicron resolution corresponding to imaging 1 million cells in 20 min. The statistical accuracy of this instrument is verified by quantitatively measuring rare cell populations with ratio ranging from 1:1 to 1:10[superscript 5].
DepartmentInstitute for Medical Engineering and Science; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Laser Biomedical Research Center; Singapore-MIT Alliance
Cytometry Part A
Choi, Heejin, Dushan N. Wadduwage, Ting Yuan Tu, Paul Matsudaira, and Peter T. C. So. “Three-Dimensional Image Cytometer Based on Widefield Structured Light Microscopy and High-Speed Remote Depth Scanning.” Cytometry 87, no. 1 (October 28, 2014): 49–60.
Author's final manuscript