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dc.contributor.authorLeslie, David J.
dc.contributor.authorHeinen, Christian
dc.contributor.authorSchramm, Frederic D.
dc.contributor.authorAakre, Christopher David
dc.contributor.authorMurray, Sean M.
dc.contributor.authorLaub, Michael T.
dc.contributor.authorJonas, Kristina
dc.contributor.authorThuring, Marietta
dc.date.accessioned2015-08-20T17:05:53Z
dc.date.available2015-08-20T17:05:53Z
dc.date.issued2015-07
dc.date.submitted2015-03
dc.identifier.issn1553-7404
dc.identifier.issn1553-7390
dc.identifier.urihttp://hdl.handle.net/1721.1/98121
dc.description.abstractBacteria can arrest their own growth and proliferation upon nutrient depletion and under various stressful conditions to ensure their survival. However, the molecular mechanisms responsible for suppressing growth and arresting the cell cycle under such conditions remain incompletely understood. Here, we identify post-transcriptional mechanisms that help enforce a cell-cycle arrest in Caulobacter crescentus following nutrient limitation and during entry into stationary phase by limiting the accumulation of DnaA, the conserved replication initiator protein. DnaA is rapidly degraded by the Lon protease following nutrient limitation. However, the rate of DnaA degradation is not significantly altered by changes in nutrient availability. Instead, we demonstrate that decreased nutrient availability downregulates dnaA translation by a mechanism involving the 5' untranslated leader region of the dnaA transcript; Lon-dependent proteolysis of DnaA then outpaces synthesis, leading to the elimination of DnaA and the arrest of DNA replication. Our results demonstrate how regulated translation and constitutive degradation provide cells a means of precisely and rapidly modulating the concentration of key regulatory proteins in response to environmental inputs.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 5R01GM082899)en_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pgen.1005342en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourcePublic Library of Scienceen_US
dc.titleNutritional Control of DNA Replication Initiation through the Proteolysis and Regulated Translation of DnaAen_US
dc.typeArticleen_US
dc.identifier.citationLeslie, David J., Christian Heinen, Frederic D. Schramm, Marietta Thüring, Christopher D. Aakre, Sean M. Murray, Michael T. Laub, and Kristina Jonas. “Nutritional Control of DNA Replication Initiation through the Proteolysis and Regulated Translation of DnaA.” Edited by William F. Burkholder. PLOS Genetics 11, no. 7 (July 2, 2015): e1005342.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorAakre, Christopher Daviden_US
dc.contributor.mitauthorLaub, Michael T.en_US
dc.relation.journalPLOS Geneticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLeslie, David J.; Heinen, Christian; Schramm, Frederic D.; Thüring, Marietta; Aakre, Christopher D.; Murray, Sean M.; Laub, Michael T.; Jonas, Kristinaen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-8288-7607
dspace.mitauthor.errortrue
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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