Improved Gene Targeting through Cell Cycle Synchronization
Author(s)
Tsakraklides, Vasiliki; Brevnova, Elena; Stephanopoulos, Gregory; Shaw, A. Joe
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Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during transformation. Using hydroxyurea-mediated cell cycle arrest, we obtained improved gene targeting rates in Yarrowia lipolytica, Arxula adeninivorans, Saccharomyces cerevisiae, Kluyveromyces lactis and Pichia pastoris demonstrating the broad applicability of the method. Hydroxyurea treatment enriches for S-phase cells that are active in homologous recombination and enables previously unattainable genomic modifications.
Date issued
2015-07Department
Massachusetts Institute of Technology. Department of Chemical EngineeringJournal
PLOS ONE
Publisher
Public Library of Science
Citation
Tsakraklides, Vasiliki, Elena Brevnova, Gregory Stephanopoulos, and A. Joe Shaw. “Improved Gene Targeting through Cell Cycle Synchronization.” Edited by Alvaro Galli. PLoS ONE 10, no. 7 (July 20, 2015): e0133434.
Version: Final published version
ISSN
1932-6203