dc.contributor.author | Ji, Xiong | |
dc.contributor.author | Abraham, Brian J. | |
dc.contributor.author | Lee, Tong Ihn | |
dc.contributor.author | Jaenisch, Rudolf | |
dc.contributor.author | Bradner, James E. | |
dc.contributor.author | Young, Richard A. | |
dc.contributor.author | Dadon, Daniel Benjamin | |
dc.date.accessioned | 2015-09-08T15:19:56Z | |
dc.date.available | 2015-09-08T15:19:56Z | |
dc.date.issued | 2015-03 | |
dc.date.submitted | 2015-01 | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.issn | 1091-6490 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/98386 | |
dc.description.abstract | More than a thousand proteins are thought to contribute to mammalian chromatin and its regulation, but our understanding of the genomic occupancy and function of most of these proteins is limited. Here we describe an approach, which we call “chromatin proteomic profiling,” to identify proteins associated with genomic regions marked by specifically modified histones. We used ChIP-MS to identify proteins associated with genomic regions marked by histones modified at specific lysine residues, including H3K27ac, H3K4me3, H3K79me2, H3K36me3, H3K9me3, and H4K20me3, in ES cells. We identified 332 known and 114 novel proteins associated with these histone-marked genomic segments. Many of the novel candidates have been implicated in various diseases, and their chromatin association may provide clues to disease mechanisms. More than 100 histone modifications have been described, so similar chromatin proteomic profiling studies should prove to be valuable for identifying many additional chromatin-associated proteins in a broad spectrum of cell types. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant HG002668) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant HG006046) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant HD045022) | en_US |
dc.language.iso | en_US | |
dc.publisher | National Academy of Sciences (U.S.) | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1073/pnas.1502971112 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | National Academy of Sciences (U.S.) | en_US |
dc.title | Chromatin proteomic profiling reveals novel proteins associated with histone-marked genomic regions | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Ji, Xiong, Daniel B. Dadon, Brian J. Abraham, Tong Ihn Lee, Rudolf Jaenisch, James E. Bradner, and Richard A. Young. “Chromatin Proteomic Profiling Reveals Novel Proteins Associated with Histone-Marked Genomic Regions.” Proceedings of the National Academy of Sciences, March 9, 2015, 201502971. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Dadon, Daniel Benjamin | en_US |
dc.contributor.mitauthor | Jaenisch, Rudolf | en_US |
dc.contributor.mitauthor | Young, Richard A. | en_US |
dc.relation.journal | Proceedings of the National Academy of Sciences | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Ji, Xiong; Dadon, Daniel B.; Abraham, Brian J.; Lee, Tong Ihn; Jaenisch, Rudolf; Bradner, James E.; Young, Richard A. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7256-3158 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8855-8647 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |