Controlled electromechanical cell stimulation on-a-chip
Author(s)Pavesi, Andrea; Adriani, Giulia; Rasponi, Marco; Zervantonakis, Ioannis K.; Fiore, Gianfranco B.; Kamm, Roger Dale; ... Show more Show less
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Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simultaneously providing mechanical, electrical, and biochemical stimulation, and subsequently extracting detailed morphological and gene-expression analysis on the cellular response. This micro-device offers the opportunity to overcome previous limitations and recreate critical elements of the in vivo microenvironment in order to investigate cellular responses to three different stimulations. The platform was validated in experiments using human bone marrow mesenchymal stem cells. These experiments demonstrated the ability for inducing changes in cell morphology, cytoskeletal fiber orientation and changes in gene expression under physiological stimuli. This novel bioengineering approach can be readily applied to various studies, especially in the fields of stem cell biology and regenerative medicine.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
Nature Publishing Group
Pavesi, Andrea, Giulia Adriani, Marco Rasponi, Ioannis K. Zervantonakis, Gianfranco B. Fiore, and Roger D. Kamm. “Controlled Electromechanical Cell Stimulation on-a-Chip.” Scientific Reports 5 (July 2, 2015): 11800.
Final published version