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dc.contributor.authorBao, Chenchen
dc.contributor.authorConde, Joao
dc.contributor.authorCurtin, James
dc.contributor.authorArtzi, Natalie
dc.contributor.authorTian, Furong
dc.contributor.authorCui, Daxiang
dc.date.accessioned2015-09-10T16:31:20Z
dc.date.available2015-09-10T16:31:20Z
dc.date.issued2015-07
dc.date.submitted2015-04
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/98440
dc.description.abstractGold nanobeacons can be used as a powerful tool for cancer theranostics. Here, we proposed a nanomaterial platform based on gold nanobeacons to detect, target and inhibit the expression of a mutant Kras gene in an in vivo murine gastric cancer model. The conjugation of fluorescently-labeled antisense DNA hairpin oligonucleotides to the surface of gold nanoparticles enables using their localized surface plasmon resonance properties to directly track the delivery to the primary gastric tumor and to lung metastatic sites. The fluorescently labeled nanobeacons reports on the interaction with the target as the fluorescent Cy3 signal is quenched by the gold nanoparticle and only emit light following conjugation to the Kras target owing to reorganization and opening of the nanobeacons, thus increasing the distance between the dye and the quencher. The systemic administration of the anti-Kras nanobeacons resulted in approximately 60% tumor size reduction and a 90% reduction in tumor vascularization. More important, the inhibition of the Kras gene expression in gastric tumors prevents the occurrence of metastasis to lung (80% reduction), increasing mice survival in more than 85%. Our developed platform can be easily adjusted to hybridize with any specific target and provide facile diagnosis and treatment for neoplastic diseases.en_US
dc.description.sponsorshipScience Foundation Ireland (Grant 11/PI/08)en_US
dc.description.sponsorshipNational Key Basic Research Program of China (973 Program) (2011CB933101)en_US
dc.description.sponsorshipNational Key Basic Research Program of China (973 Program) (2015CB931802)en_US
dc.description.sponsorshipNational Natural Scientific Fund (China) (81225010)en_US
dc.description.sponsorship863 Project of China (2012AA022703)en_US
dc.description.sponsorship863 Project of China (2014AA020700)en_US
dc.description.sponsorshipShanghai Science and Technology Fund (13NM1401500)en_US
dc.description.sponsorshipMarie Curie International Fellowship (FP7-PEOPLE-2013-IOF, Project 626386)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep12297en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleBioresponsive antisense DNA gold nanobeacons as a hybrid in vivo theranostics platform for the inhibition of cancer cells and metastasisen_US
dc.typeArticleen_US
dc.identifier.citationBao, Chenchen, Joao Conde, James Curtin, Natalie Artzi, Furong Tian, and Daxiang Cui. “Bioresponsive Antisense DNA Gold Nanobeacons as a Hybrid in Vivo Theranostics Platform for the Inhibition of Cancer Cells and Metastasis.” Scientific Reports 5 (July 20, 2015): 12297.en_US
dc.contributor.departmentInstitute for Medical Engineering and Scienceen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.mitauthorConde, Joaoen_US
dc.contributor.mitauthorArtzi, Natalieen_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsBao, Chenchen; Conde, Joao; Curtin, James; Artzi, Natalie; Tian, Furong; Cui, Daxiangen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-8422-6792
mit.licensePUBLISHER_CCen_US


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