Show simple item record

dc.contributor.authorSakurai, Takeshi
dc.contributor.authorMitsuno, Hidefumi
dc.contributor.authorMikami, Akihisa
dc.contributor.authorUchino, Keiro
dc.contributor.authorTabuchi, Masashi
dc.contributor.authorZhang, Feng
dc.contributor.authorSezutsu, Hideki
dc.contributor.authorKanzaki, Ryohei
dc.date.accessioned2015-09-10T16:35:48Z
dc.date.available2015-09-10T16:35:48Z
dc.date.issued2015-06
dc.date.submitted2015-02
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/98441
dc.description.abstractMale moths use species-specific sex pheromones to identify and orientate toward conspecific females. Odorant receptors (ORs) for sex pheromone substances have been identified as sex pheromone receptors in various moth species. However, direct in vivo evidence linking the functional role of these ORs with behavioural responses is lacking. In the silkmoth, Bombyx mori, female moths emit two sex pheromone components, bombykol and bombykal, but only bombykol elicits sexual behaviour in male moths. A sex pheromone receptor BmOR1 is specifically tuned to bombykol and is expressed in specialized olfactory receptor neurons (ORNs) in the pheromone sensitive long sensilla trichodea of male silkmoth antennae. Here, we show that disruption of the BmOR1 gene, mediated by transcription activator-like effector nucleases (TALENs), completely removes ORN sensitivity to bombykol and corresponding pheromone-source searching behaviour in male moths. Furthermore, transgenic rescue of BmOR1 restored normal behavioural responses to bombykol. Our results demonstrate that BmOR1 is required for the physiological and behavioural response to bombykol, demonstrating that it is the receptor that mediates sex pheromone responses in male silkmoths. This study provides the first direct evidence that a member of the sex pheromone receptor family in moth species mediates conspecific sex pheromone information for sexual behaviour.en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep11001en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleTargeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmothen_US
dc.typeArticleen_US
dc.identifier.citationSakurai, Takeshi, Hidefumi Mitsuno, Akihisa Mikami, Keiro Uchino, Masashi Tabuchi, Feng Zhang, Hideki Sezutsu, and Ryohei Kanzaki. “Targeted Disruption of a Single Sex Pheromone Receptor Gene Completely Abolishes in Vivo Pheromone Response in the Silkmoth.” Scientific Reports 5 (June 5, 2015): 11001.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.mitauthorZhang, Fengen_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSakurai, Takeshi; Mitsuno, Hidefumi; Mikami, Akihisa; Uchino, Keiro; Tabuchi, Masashi; Zhang, Feng; Sezutsu, Hideki; Kanzaki, Ryoheien_US
dc.identifier.orcidhttps://orcid.org/0000-0003-2782-2509
mit.licensePUBLISHER_CCen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record