dc.contributor.author | Cao, Bo | |
dc.contributor.author | Cheng, Qiuxiang | |
dc.contributor.author | Gu, Chen | |
dc.contributor.author | Yao, Fen | |
dc.contributor.author | DeMott, Michael S. | |
dc.contributor.author | Zheng, Xiaoqing | |
dc.contributor.author | Deng, Zixin | |
dc.contributor.author | Dedon, Peter C. | |
dc.contributor.author | You, Delin | |
dc.date.accessioned | 2015-10-15T13:41:39Z | |
dc.date.available | 2015-10-15T13:41:39Z | |
dc.date.issued | 2014-07 | |
dc.identifier.issn | 0950382X | |
dc.identifier.issn | 1365-2958 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/99340 | |
dc.description.abstract | Prokaryotes protect their genomes from foreign DNA with a diversity of defence mechanisms, including a widespread restriction–modification (R–M) system involving phosphorothioate (PT) modification of the DNA backbone. Unlike classical R–M systems, highly partial PT modification of consensus motifs in bacterial genomes suggests an unusual mechanism of PT-dependent restriction. In Salmonella enterica, PT modification is mediated by four genes dptB–E, while restriction involves additional three genes dptF–H. Here, we performed a series of studies to characterize the PT-dependent restriction, and found that it presented several features distinct with traditional R–M systems. The presence of restriction genes in a PT-deficient mutant was not lethal, but instead resulted in several pathological phenotypes. Subsequent transcriptional profiling revealed the expression of > 600 genes was affected by restriction enzymes in cells lacking PT, including induction of bacteriophage, SOS response and DNA repair-related genes. These transcriptional responses are consistent with the observation that restriction enzymes caused extensive DNA cleavage in the absence of PT modifications in vivo. However, overexpression of restriction genes was lethal to the host in spite of the presence PT modifications. These results point to an unusual mechanism of PT-dependent DNA cleavage by restriction enzymes in the face of partial PT modification. | en_US |
dc.description.sponsorship | National Natural Science Foundation (China) (Grant 31170085) | en_US |
dc.description.sponsorship | National Natural Science Foundation (China) (Grant 31070058) | en_US |
dc.description.sponsorship | Ministry of Science and Technology of the People's Republic of China (973 and 863 Programs) | en_US |
dc.description.sponsorship | China Scholarship Council | en_US |
dc.description.sponsorship | National Science Foundation (U.S.) (Grant CHE-1019990) | en_US |
dc.description.sponsorship | Shanghai Municipal Council of Science and Technology. Shanghai Pujiang Program (Grant 12PJD021) | en_US |
dc.language.iso | en_US | |
dc.publisher | Wiley Blackwell | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1111/mmi.12692 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in Salmonella | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Cao, Bo, Qiuxiang Cheng, Chen Gu, Fen Yao, Michael S. DeMott, Xiaoqing Zheng, Zixin Deng, Peter C. Dedon, and Delin You. “ Pathological Phenotypes and in Vivo DNA Cleavage by Unrestrained Activity of a Phosphorothioate-Based Restriction System in S Almonella .” Molecular Microbiology 93, no. 4 (July 23, 2014): 776–785. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Center for Environmental Health Sciences | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.mitauthor | Cao, Bo | en_US |
dc.contributor.mitauthor | Gu, Chen | en_US |
dc.contributor.mitauthor | DeMott, Michael S. | en_US |
dc.contributor.mitauthor | Dedon, Peter C. | en_US |
dc.relation.journal | Molecular Microbiology | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Cao, Bo; Cheng, Qiuxiang; Gu, Chen; Yao, Fen; DeMott, Michael S.; Zheng, Xiaoqing; Deng, Zixin; Dedon, Peter C.; You, Delin | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-1615-1578 | |
dc.identifier.orcid | https://orcid.org/0000-0003-0011-3067 | |
dc.identifier.orcid | https://orcid.org/0000-0001-9920-2080 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |