dc.contributor.author | Belair, David G. | |
dc.contributor.author | Molenda, James A. | |
dc.contributor.author | Vickerman, Vernella | |
dc.contributor.author | Lewis, Rachel | |
dc.contributor.author | Daigh, Christine | |
dc.contributor.author | Hansen, Tyler D. | |
dc.contributor.author | Mann, David A. | |
dc.contributor.author | Thomson, James A. | |
dc.contributor.author | Griffith, Linda G. | |
dc.contributor.author | Schwartz, Michael P. | |
dc.contributor.author | Murphy, William L. | |
dc.contributor.author | Whisler, Jordan Ari | |
dc.contributor.author | Kamm, Roger Dale | |
dc.contributor.author | Valdez Macias, Jorge Luis | |
dc.contributor.author | Velazquez, Jeremy J. | |
dc.date.accessioned | 2015-10-22T13:47:10Z | |
dc.date.available | 2015-10-22T13:47:10Z | |
dc.date.issued | 2014-09 | |
dc.identifier.issn | 1550-8943 | |
dc.identifier.issn | 1558-6804 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/99408 | |
dc.description.abstract | Here we describe a strategy to model blood vessel development using a well-defined induced pluripotent stem cell-derived endothelial cell type (iPSC-EC) cultured within engineered platforms that mimic the 3D microenvironment. The iPSC-ECs used here were first characterized by expression of endothelial markers and functional properties that included VEGF responsiveness, TNF-α-induced upregulation of cell adhesion molecules (MCAM/CD146; ICAM1/CD54), thrombin-dependent barrier function, shear stress-induced alignment, and 2D and 3D capillary-like network formation in Matrigel. The iPSC-ECs also formed 3D vascular networks in a variety of engineering contexts, yielded perfusable, interconnected lumen when co-cultured with primary human fibroblasts, and aligned with flow in microfluidics devices. iPSC-EC function during tubule network formation, barrier formation, and sprouting was consistent with that of primary ECs, and the results suggest a VEGF-independent mechanism for sprouting, which is relevant to therapeutic anti-angiogenesis strategies. Our combined results demonstrate the feasibility of using a well-defined, stable source of iPSC-ECs to model blood vessel formation within a variety of contexts using standard in vitro formats. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (NIH 1UH2 TR000506-01) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (3UH2 TR000506-02S1) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (T32 HL007936-12) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (RO1 HL093282) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (R21 EB016381-01) | en_US |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1007/s12015-014-9549-5 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Belair, David G., Jordan A. Whisler, Jorge Valdez, Jeremy Velazquez, James A. Molenda, Vernella Vickerman, Rachel Lewis, et al. “Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells.” Stem Cell Reviews and Reports 11, no. 3 (September 5, 2014): 511–525. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Mechanical Engineering | en_US |
dc.contributor.mitauthor | Whisler, Jordan Ari | en_US |
dc.contributor.mitauthor | Kamm, Roger Dale | en_US |
dc.contributor.mitauthor | Valdez Macias, Jorge Luis | en_US |
dc.contributor.mitauthor | Velazquez, Jeremy J. | en_US |
dc.contributor.mitauthor | Griffith, Linda G. | en_US |
dc.relation.journal | Stem Cell Reviews and Reports | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Belair, David G.; Whisler, Jordan A.; Valdez, Jorge; Velazquez, Jeremy; Molenda, James A.; Vickerman, Vernella; Lewis, Rachel; Daigh, Christine; Hansen, Tyler D.; Mann, David A.; Thomson, James A.; Griffith, Linda G.; Kamm, Roger D.; Schwartz, Michael P.; Murphy, William L. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3299-9424 | |
dc.identifier.orcid | https://orcid.org/0000-0002-6673-087X | |
dc.identifier.orcid | https://orcid.org/0000-0002-1801-5548 | |
dc.identifier.orcid | https://orcid.org/0000-0002-7232-304X | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |