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dc.contributor.authorBelair, David G.
dc.contributor.authorMolenda, James A.
dc.contributor.authorVickerman, Vernella
dc.contributor.authorLewis, Rachel
dc.contributor.authorDaigh, Christine
dc.contributor.authorHansen, Tyler D.
dc.contributor.authorMann, David A.
dc.contributor.authorThomson, James A.
dc.contributor.authorGriffith, Linda G.
dc.contributor.authorSchwartz, Michael P.
dc.contributor.authorMurphy, William L.
dc.contributor.authorWhisler, Jordan Ari
dc.contributor.authorKamm, Roger Dale
dc.contributor.authorValdez Macias, Jorge Luis
dc.contributor.authorVelazquez, Jeremy J.
dc.date.accessioned2015-10-22T13:47:10Z
dc.date.available2015-10-22T13:47:10Z
dc.date.issued2014-09
dc.identifier.issn1550-8943
dc.identifier.issn1558-6804
dc.identifier.urihttp://hdl.handle.net/1721.1/99408
dc.description.abstractHere we describe a strategy to model blood vessel development using a well-defined induced pluripotent stem cell-derived endothelial cell type (iPSC-EC) cultured within engineered platforms that mimic the 3D microenvironment. The iPSC-ECs used here were first characterized by expression of endothelial markers and functional properties that included VEGF responsiveness, TNF-α-induced upregulation of cell adhesion molecules (MCAM/CD146; ICAM1/CD54), thrombin-dependent barrier function, shear stress-induced alignment, and 2D and 3D capillary-like network formation in Matrigel. The iPSC-ECs also formed 3D vascular networks in a variety of engineering contexts, yielded perfusable, interconnected lumen when co-cultured with primary human fibroblasts, and aligned with flow in microfluidics devices. iPSC-EC function during tubule network formation, barrier formation, and sprouting was consistent with that of primary ECs, and the results suggest a VEGF-independent mechanism for sprouting, which is relevant to therapeutic anti-angiogenesis strategies. Our combined results demonstrate the feasibility of using a well-defined, stable source of iPSC-ECs to model blood vessel formation within a variety of contexts using standard in vitro formats.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH 1UH2 TR000506-01)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (3UH2 TR000506-02S1)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (T32 HL007936-12)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (RO1 HL093282)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (R21 EB016381-01)en_US
dc.language.isoen_US
dc.publisherSpringer-Verlagen_US
dc.relation.isversionofhttp://dx.doi.org/10.1007/s12015-014-9549-5en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleHuman Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cellsen_US
dc.typeArticleen_US
dc.identifier.citationBelair, David G., Jordan A. Whisler, Jorge Valdez, Jeremy Velazquez, James A. Molenda, Vernella Vickerman, Rachel Lewis, et al. “Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells.” Stem Cell Reviews and Reports 11, no. 3 (September 5, 2014): 511–525.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorWhisler, Jordan Arien_US
dc.contributor.mitauthorKamm, Roger Daleen_US
dc.contributor.mitauthorValdez Macias, Jorge Luisen_US
dc.contributor.mitauthorVelazquez, Jeremy J.en_US
dc.contributor.mitauthorGriffith, Linda G.en_US
dc.relation.journalStem Cell Reviews and Reportsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsBelair, David G.; Whisler, Jordan A.; Valdez, Jorge; Velazquez, Jeremy; Molenda, James A.; Vickerman, Vernella; Lewis, Rachel; Daigh, Christine; Hansen, Tyler D.; Mann, David A.; Thomson, James A.; Griffith, Linda G.; Kamm, Roger D.; Schwartz, Michael P.; Murphy, William L.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-3299-9424
dc.identifier.orcidhttps://orcid.org/0000-0002-6673-087X
dc.identifier.orcidhttps://orcid.org/0000-0002-1801-5548
dc.identifier.orcidhttps://orcid.org/0000-0002-7232-304X
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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