dc.contributor.author | Castellarnau, Marc | |
dc.contributor.author | Su, Hao-wei | |
dc.contributor.author | Tokatlian, Talar | |
dc.contributor.author | Voldman, Joel | |
dc.contributor.author | Szeto, Gregory | |
dc.contributor.author | Love, John C | |
dc.contributor.author | Irvine, Darrell J | |
dc.date.accessioned | 2015-10-23T17:49:49Z | |
dc.date.available | 2015-10-23T17:49:49Z | |
dc.date.issued | 2014-09 | |
dc.identifier.issn | 16136810 | |
dc.identifier.issn | 1613-6829 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/99439 | |
dc.description.abstract | This study presents stochastic particle barcoding (SPB), a method for tracking cell identity across bioanalytical platforms. In this approach, single cells or small collections of cells are co-encapsulated within an enzymatically-degradable hydrogel block along with a random collection of fluorescent beads, whose number, color, and position encode the identity of the cell, enabling samples to be transferred in bulk between single-cell assay platforms without losing the identity of individual cells. The application of SPB is demonstrated for transferring cells from a subnanoliter protein secretion/phenotyping array platform into a microtiter plate, with re-identification accuracies in the plate assay of 96±2%. Encapsulated cells are recovered by digesting the hydrogel, allowing subsequent genotyping and phenotyping of cell lysates. Finally, a model scaling is developed to illustrate how different parameters affect the accuracy of SPB and to motivate scaling of the method to thousands of unique blocks. | en_US |
dc.description.sponsorship | Ragon Institute of MGH, MIT and Harvard | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (Koch Institute Support (Core) Grant P30-CA14051) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.). Ruth L. Kirschstein National Research Service Award (1F32CA180586) | en_US |
dc.language.iso | en_US | |
dc.publisher | Wiley Blackwell | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1002/smll.201401369 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Stochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Castellarnau, M., G. L. Szeto, H.-W. Su, T. Tokatlian, J. C. Love, D. J. Irvine, and J. Voldman. “Stochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis.” Small 11, no. 4 (September 2, 2014): 489–498. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
dc.contributor.department | Ragon Institute of MGH, MIT and Harvard | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.mitauthor | Castellarnau, Marc | en_US |
dc.contributor.mitauthor | Szeto, Gregory Lee | en_US |
dc.contributor.mitauthor | Su, Hao-wei | en_US |
dc.contributor.mitauthor | Tokatlian, Talar | en_US |
dc.contributor.mitauthor | Love, J. Christopher | en_US |
dc.contributor.mitauthor | Irvine, Darrell J. | en_US |
dc.contributor.mitauthor | Voldman, Joel | en_US |
dc.relation.journal | Small | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Castellarnau, M.; Szeto, G. L.; Su, H.-W.; Tokatlian, T.; Love, J. C.; Irvine, D. J.; Voldman, J. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-9028-3117 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8898-2296 | |
dc.identifier.orcid | https://orcid.org/0000-0001-7604-1333 | |
dc.identifier.orcid | https://orcid.org/0000-0003-0921-3144 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8452-6752 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |