Efficient CRISPR/Cas9-mediated genome editing in P. falciparum
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Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum
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Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥50–100%) gene disruption frequencies within the usual time frame for generating transgenic parasites.
Date issued
2014-08Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MITJournal
Nature Methods
Publisher
Nature Publishing Group
Citation
Wagner, Jeffrey C, Randall J Platt, Stephen J Goldfless, Feng Zhang, and Jacquin C Niles. “Efficient CRISPR-Cas9–mediated Genome Editing in Plasmodium Falciparum.” Nat Meth 11, no. 9 (August 10, 2014): 915–918.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105