dc.contributor.author | Tamsir, Alvin | |
dc.contributor.author | Clancy, Kevin | |
dc.contributor.author | Peterson, Todd | |
dc.contributor.author | Stanton, Brynne C. | |
dc.contributor.author | Nielsen, Alec Andrew | |
dc.contributor.author | Voigt, Christopher A. | |
dc.date.accessioned | 2015-10-30T15:51:51Z | |
dc.date.available | 2015-10-30T15:51:51Z | |
dc.date.issued | 2013-12 | |
dc.date.submitted | 2013-08 | |
dc.identifier.issn | 1552-4450 | |
dc.identifier.issn | 1552-4469 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/99526 | |
dc.description.abstract | Genetic circuits perform computational operations based on interactions between freely diffusing molecules within a cell. When transcription factors are combined to build a circuit, unintended interactions can disrupt its function. Here, we apply 'part mining' to build a library of 73 TetR-family repressors gleaned from prokaryotic genomes. The operators of a subset were determined using an in vitro method, and this information was used to build synthetic promoters. The promoters and repressors were screened for cross-reactions. Of these, 16 were identified that both strongly repress their cognate promoter (5- to 207-fold) and exhibit minimal interactions with other promoters. Each repressor-promoter pair was converted to a NOT gate and characterized. Used as a set of 16 NOT/NOR gates, there are >10[superscript 54] circuits that could be built by changing the pattern of input and output promoters. This represents a large set of compatible gates that can be used to construct user-defined circuits. | en_US |
dc.description.sponsorship | United States. Air Force Office of Scientific Research (Award FA9550-11-C-0028) | en_US |
dc.description.sponsorship | American Society for Engineering Education. National Defense Science and Engineering Graduate Fellowship (32 CFR 168a) | en_US |
dc.description.sponsorship | United States. Defense Advanced Research Projects Agency. Chronical of Lineage Indicative of Origins (N66001-12-C-4016) | en_US |
dc.description.sponsorship | United States. Office of Naval Research (N00014-13-1-0074) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (GM095765) | en_US |
dc.description.sponsorship | National Science Foundation (U.S.). Synthetic Biology Engineering Research Center (SA5284-11210) | en_US |
dc.language.iso | en_US | |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/nchembio.1411 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Genomic mining of prokaryotic repressors for orthogonal logic gates | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Stanton, Brynne C, Alec A K Nielsen, Alvin Tamsir, Kevin Clancy, Todd Peterson, and Christopher A Voigt. “Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates.” Nat Chem Biol 10, no. 2 (December 8, 2013): 99–105. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Synthetic Biology Center | en_US |
dc.contributor.mitauthor | Stanton, Brynne C. | en_US |
dc.contributor.mitauthor | Nielsen, Alec Andrew | en_US |
dc.contributor.mitauthor | Voigt, Christopher A. | en_US |
dc.relation.journal | Nature Chemical Biology | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Stanton, Brynne C; Nielsen, Alec A K; Tamsir, Alvin; Clancy, Kevin; Peterson, Todd; Voigt, Christopher A | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-0844-4776 | |
dc.identifier.orcid | https://orcid.org/0000-0003-2171-8460 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |