CRISPR transcriptional repression devices and layered circuits in mammalian cells
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A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
Date issued
2014-05Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Synthetic Biology CenterJournal
Nature Methods
Publisher
Nature Publishing Group
Citation
Kiani, Samira, Jacob Beal, Mohammad R Ebrahimkhani, Jin Huh, Richard N Hall, Zhen Xie, Yinqing Li, and Ron Weiss. “CRISPR Transcriptional Repression Devices and Layered Circuits in Mammalian Cells.” Nat Meth 11, no. 7 (May 5, 2014): 723–726.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105