Highly efficient Cas9-mediated transcriptional programming

Highly efficient Cas9-mediated transcriptional programming

Author: Chavez, Alejandro; Scheiman, Jonathan; Vora, Suhani; Pruitt, Benjamin W; Tuttle, Marcelle; P R Iyer, Eswar; Lin, Shuailiang; Kiani, Samira; Guzman, Christopher D; Wiegand, Daniel J; Ter-Ovanesyan, Dmitry; Braff, Jonathan L; Davidsohn, Noah; Housden, Benjamin E; Perrimon, Norbert; Weiss, Ron; Aach, John; Collins, James J; Church, George M

Citable URI: http://hdl.handle.net/1721.1/99529

Department: Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Synthetic Biology Center; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Institute for Medical Engineering and Science

Publisher: Nature Publishing Group

Date Issued: 2015-03

Abstract:

The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).

URI: http://hdl.handle.net/1721.1/99529

ISSN: 1548-7091
1548-7105

Citation: Chavez, Alejandro, Jonathan Scheiman, Suhani Vora, Benjamin W Pruitt, Marcelle Tuttle, Eswar P R Iyer, Shuailiang Lin, et al. “Highly Efficient Cas9-Mediated Transcriptional Programming.” Nat Meth 12, no. 4 (March 2, 2015): 326–328.

Version: Author's final manuscript

Terms of Use: Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.

Published As: http://dx.doi.org/10.1038/nmeth.3312

Journal: Nature Methods

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