Highly efficient Cas9-mediated transcriptional programming
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Author(s)
Chavez, Alejandro
Scheiman, Jonathan
Tuttle, Marcelle
Lin, Shuailiang
Kiani, Samira
Ter-Ovanesyan, Dmitry
Davidsohn, Noah
Perrimon, Norbert
Weiss, Ron
Aach, John
Date Issued
March 2015
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Chavez, Alejandro, Jonathan Scheiman, Suhani Vora, Benjamin W Pruitt, Marcelle Tuttle, Eswar P R Iyer, Shuailiang Lin, et al. “Highly Efficient Cas9-Mediated Transcriptional Programming.” Nat Meth 12, no. 4 (March 2, 2015): 326–328.
Version
Author's final manuscript
Abstract
The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Synthetic Biology Center
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmeth.3312
