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dc.contributor.authorHar, Jia Y.
dc.contributor.authorHelbig, Tim
dc.contributor.authorLim, Ju H.
dc.contributor.authorFernando, Samodha C.
dc.contributor.authorReitzel, Adam M.
dc.contributor.authorPenn, Kevin
dc.contributor.authorThompson, Janelle Renee
dc.date.accessioned2015-11-03T18:00:34Z
dc.date.available2015-11-03T18:00:34Z
dc.date.issued2015-09
dc.date.submitted2014-05
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/1721.1/99685
dc.description.abstractWe have characterized the molecular and genomic diversity of the microbiota of the starlet sea anemone Nematostella vectensis, a cnidarian model for comparative developmental and functional biology and a year-round inhabitant of temperate salt marshes. Molecular phylogenetic analysis of 16S rRNA gene clone libraries revealed four ribotypes associated with N. vectensis at multiple locations and times. These associates include two novel ribotypes within the ε-Proteobacterial order Campylobacterales and the Spirochetes, respectively, each sharing <85% identity with cultivated strains, and two γ-Proteobacterial ribotypes sharing >99% 16S rRNA identity with Endozoicomonas elysicola and Pseudomonas oleovorans, respectively. Species-specific PCR revealed that these populations persisted in N. vectensis asexually propagated under laboratory conditions. cDNA indicated expression of the Campylobacterales and Endozoicomonas 16S rRNA in anemones from Sippewissett Marsh, MA. A collection of bacteria from laboratory raised N. vectensis was dominated by isolates from P. oleovorans and Rhizobium radiobacter. Isolates from field-collected anemones revealed an association with Limnobacter and Stappia isolates. Genomic DNA sequencing was carried out on 10 cultured bacterial isolates representing field- and laboratory-associates, i.e., Limnobacter spp., Stappia spp., P. oleovorans and R. radiobacter. Genomes contained multiple genes identified as virulence (host-association) factors while S. stellulata and L. thiooxidans genomes revealed pathways for mixotrophic sulfur oxidation. A pilot metatranscriptome of laboratory-raised N. vectensis was compared to the isolate genomes and indicated expression of ORFs from L. thiooxidans with predicted functions of motility, nutrient scavenging (Fe and P), polyhydroxyalkanoate synthesis for carbon storage, and selective permeability (porins). We hypothesize that such activities may mediate acclimation and persistence of bacteria in a N. vectensis holobiont defined by both internal and external gradients of chemicals and nutrients in a dynamic coastal habitat.en_US
dc.description.sponsorshipMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.description.sponsorshipNational Science Foundation (U.S.). Graduate Research Fellowshipen_US
dc.description.sponsorshipNational Research Foundation of Korea (Fellowship)en_US
dc.language.isoen_US
dc.publisherFrontiers Research Foundationen_US
dc.relation.isversionofhttp://dx.doi.org/10.3389/fmicb.2015.00818en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceFrontiers Research Foundationen_US
dc.titleMicrobial diversity and activity in the Nematostella vectensis holobiont: insights from 16S rRNA gene sequencing, isolate genomes, and a pilot-scale survey of gene expressionen_US
dc.typeArticleen_US
dc.identifier.citationHar, Jia Y., Tim Helbig, Ju H. Lim, Samodha C. Fernando, Adam M. Reitzel, Kevin Penn, and Janelle R. Thompson. “Microbial Diversity and Activity in the Nematostella Vectensis Holobiont: Insights from 16S rRNA Gene Sequencing, Isolate Genomes, and a Pilot-Scale Survey of Gene Expression.” Frontiers in Microbiology 6 (September 2, 2015).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.contributor.mitauthorHar, Jia Y.en_US
dc.contributor.mitauthorHelbig, Timen_US
dc.contributor.mitauthorLim, Ju H.en_US
dc.contributor.mitauthorFernando, Samodha C.en_US
dc.contributor.mitauthorPenn, Kevinen_US
dc.contributor.mitauthorThompson, Janelle Reneeen_US
dc.relation.journalFrontiers in Microbiologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHar, Jia Y.; Helbig, Tim; Lim, Ju H.; Fernando, Samodha C.; Reitzel, Adam M.; Penn, Kevin; Thompson, Janelle R.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-1123-8458
mit.licensePUBLISHER_CCen_US


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