Genome-wide dissection of microRNA functions and cotargeting networks using gene-set signatures
Author(s)
Tsang, John S.; Ebert, Margaret S.; van Oudenaarden, Alexander![Thumbnail](/bitstream/handle/1721.1/76631/van%20Oudenaarden_Genome-wide.pdf.jpg?sequence=4&isAllowed=y)
Downloadvan Oudenaarden_Genome-wide.pdf (566.2Kb)
OPEN_ACCESS_POLICY
Open Access Policy
Creative Commons Attribution-Noncommercial-Share Alike
Terms of use
Metadata
Show full item recordAbstract
MicroRNAs are emerging as important regulators of diverse biological processes and pathologies in animals and plants. Though hundreds of human microRNAs are known, only a few have known functions. Here, we predict human microRNA functions by using a new method that systematically assesses the statistical enrichment of several microRNA-targeting signatures in annotated gene sets such as signaling networks and protein complexes. Some of our top predictions are supported by published experiments, yet many are entirely new or provide mechanistic insights to known phenotypes. Our results indicate that coordinated microRNA targeting of closely connected genes is prevalent across pathways. We use the same method to infer which microRNAs regulate similar targets and provide the first genome-wide evidence of pervasive cotargeting, in which a handful of “hub” microRNAs are involved in a majority of cotargeting relationships. Our method and analyses pave the way to systematic discovery of microRNA functions.
Date issued
2010-04Department
Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Physics; Koch Institute for Integrative Cancer Research at MITJournal
Molecular Cell
Publisher
Elsevier
Citation
Tsang, John S., Margaret S. Ebert, and Alexander van Oudenaarden. “Genome-wide Dissection of MicroRNA Functions and Cotargeting Networks Using Gene Set Signatures.” Molecular Cell 38.1 (2010): 140–153.
Version: Author's final manuscript
ISSN
1097-2765