Effect of Nanoparticle Conjugation on Gene Silencing by RNA Interference
Author(s)
Singh, Neetu; Agrawal, Amit; Leung, Anthony; Sharp, Phillip A.; Bhatia, Sangeeta N.
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RNA interference (RNAi) is a cellular process whereby the silencing of a particular gene is mediated by short RNAs (siRNAs). Although siRNAs have great therapeutic potential, cellular delivery has been a challenge. Nanoparticle-siRNA conjugates have emerged as potential delivery vehicles; however, reports describing the effects of nanoparticle conjugation on RISC incorporation and subsequent gene silencing have been mixed. In this report, we have systematically evaluated the effect of siRNA coupling strategies using a model nanoparticle system with varying conjugation schemes. We show that the accessibility of the siRNA linked to the nanoparticle and the lability of the cross-linker are critical for efficient gene knockdown.
Date issued
2010-06Department
Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Koch Institute for Integrative Cancer Research at MITJournal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Singh, Neetu et al. “Effect of Nanoparticle Conjugation on Gene Silencing by RNA Interference.” Journal of the American Chemical Society 132.24 (2010) : 8241-8243. Copyright © 2010 American Chemical Society
Version: Final published version
ISSN
0002-7863
1520-5126