dc.contributor.author | Singh, Neetu | |
dc.contributor.author | Agrawal, Amit | |
dc.contributor.author | Leung, Anthony | |
dc.contributor.author | Sharp, Phillip A. | |
dc.contributor.author | Bhatia, Sangeeta N. | |
dc.date.accessioned | 2011-08-31T17:49:28Z | |
dc.date.available | 2011-08-31T17:49:28Z | |
dc.date.issued | 2010-06 | |
dc.date.submitted | 2010-03 | |
dc.identifier.issn | 0002-7863 | |
dc.identifier.issn | 1520-5126 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/65573 | |
dc.description.abstract | RNA interference (RNAi) is a cellular process whereby the silencing of a particular gene is mediated by short RNAs (siRNAs). Although siRNAs have great therapeutic potential, cellular delivery has been a challenge. Nanoparticle-siRNA conjugates have emerged as potential delivery vehicles; however, reports describing the effects of nanoparticle conjugation on RISC incorporation and subsequent gene silencing have been mixed. In this report, we have systematically evaluated the effect of siRNA coupling strategies using a model nanoparticle system with varying conjugation schemes. We show that the accessibility of the siRNA linked to the nanoparticle and the lability of the cross-linker are critical for efficient gene knockdown. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant R01-CA124427) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant U54-CA119349) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant U54- CA119335) | en_US |
dc.description.sponsorship | David H. Koch Cancer Research Fund | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant R01-CA133404) | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (Grant PO1- CA42063) | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (Grant P30-CA14051) | en_US |
dc.language.iso | en_US | |
dc.publisher | American Chemical Society | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1021/ja102132e | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | ACS | en_US |
dc.title | Effect of Nanoparticle Conjugation on Gene Silencing by RNA Interference | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Singh, Neetu et al. “Effect of Nanoparticle Conjugation on Gene Silencing by RNA Interference.” Journal of the American Chemical Society 132.24 (2010) : 8241-8243. Copyright © 2010 American Chemical Society | en_US |
dc.contributor.department | Harvard University--MIT Division of Health Sciences and Technology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.approver | Bhatia, Sangeeta N. | |
dc.contributor.mitauthor | Singh, Neetu | |
dc.contributor.mitauthor | Agrawal, Amit | |
dc.contributor.mitauthor | Leung, Anthony | |
dc.contributor.mitauthor | Sharp, Phillip A. | |
dc.contributor.mitauthor | Bhatia, Sangeeta N. | |
dc.relation.journal | Journal of the American Chemical Society | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Singh, Neetu; Agrawal, Amit; Leung, Anthony K. L.; Sharp, Phillip A.; Bhatia, Sangeeta N. | en |
dc.identifier.orcid | https://orcid.org/0000-0003-1465-1691 | |
dc.identifier.orcid | https://orcid.org/0000-0002-1293-2097 | |
dspace.mitauthor.error | true | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |