An Amyloid Organelle: Solid State NMR Evidence for Cross-Beta Assembly of Gas Vesicles
Author(s)
Bayro, Marvin J.; Daviso, Eugenio; Belenky, Marina; Griffin, Robert Guy; Herzfeld, Judith
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Functional amyloids have been identified in a wide range of organisms, taking on a variety of biological roles and being controlled by remarkable mechanisms of directed assembly. Here, we report that amyloid fibrils constitute the ribs of the buoyancy organelles of Anabaena flos-aquae. The walls of these gas-filled vesicles are known to comprise a single protein, GvpA, arranged in a low pitch helix. However, the tertiary and quaternary structures have been elusive. Using solid-state NMR correlation spectroscopy we find detailed evidence for an extended cross-β structure. This amyloid assembly helps to account for the strength and amphiphilic properties of the vesicle wall. Buoyancy organelles thus dramatically extend the scope of known functional amyloids.
Date issued
2012-01Department
Massachusetts Institute of Technology. Department of Chemistry; Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)Journal
Journal of Biological Chemistry
Publisher
American Society for Biochemistry and Molecular Biology
Citation
Bayro, M. J. et al. “An Amyloid Organelle, Solid-state NMR Evidence for Cross- Assembly of Gas Vesicles.” Journal of Biological Chemistry 287.5 (2011): 3479–3484. Web.
Version: Author's final manuscript
ISSN
0021-9258
1083-351X