dc.contributor.author | Bayro, Marvin J. | |
dc.contributor.author | Daviso, Eugenio | |
dc.contributor.author | Belenky, Marina | |
dc.contributor.author | Griffin, Robert Guy | |
dc.contributor.author | Herzfeld, Judith | |
dc.date.accessioned | 2012-11-08T17:18:46Z | |
dc.date.available | 2012-11-08T17:18:46Z | |
dc.date.issued | 2012-01 | |
dc.date.submitted | 2011-11 | |
dc.identifier.issn | 0021-9258 | |
dc.identifier.issn | 1083-351X | |
dc.identifier.uri | http://hdl.handle.net/1721.1/74602 | |
dc.description.abstract | Functional amyloids have been identified in a wide range of organisms, taking on a variety of biological roles and being controlled by remarkable mechanisms of directed assembly. Here, we report that amyloid fibrils constitute the ribs of the buoyancy organelles of Anabaena flos-aquae. The walls of these gas-filled vesicles are known to comprise a single protein, GvpA, arranged in a low pitch helix. However, the tertiary and quaternary structures have been elusive. Using solid-state NMR correlation spectroscopy we find detailed evidence for an extended cross-β structure. This amyloid assembly helps to account for the strength and amphiphilic properties of the vesicle wall. Buoyancy organelles thus dramatically extend the scope of known functional amyloids. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (grant EB001035) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (grant EB003151) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (grant EB002026) | en_US |
dc.language.iso | en_US | |
dc.publisher | American Society for Biochemistry and Molecular Biology | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1074/jbc.M111.313049 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike 3.0 | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
dc.source | Prof. Griffin via Erja Kajosalo | en_US |
dc.title | An Amyloid Organelle: Solid State NMR Evidence for Cross-Beta Assembly of Gas Vesicles | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Bayro, M. J. et al. “An Amyloid Organelle, Solid-state NMR Evidence for Cross- Assembly of Gas Vesicles.” Journal of Biological Chemistry 287.5 (2011): 3479–3484. Web. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
dc.contributor.department | Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology) | en_US |
dc.contributor.approver | Griffin, Robert Guy | |
dc.contributor.mitauthor | Griffin, Robert Guy | |
dc.contributor.mitauthor | Daviso, Eugenio | |
dc.contributor.mitauthor | Bayro, Marvin J. | |
dc.relation.journal | Journal of Biological Chemistry | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Bayro, M. J.; Daviso, E.; Belenky, M.; Griffin, R. G.; Herzfeld, J. | en |
dc.identifier.orcid | https://orcid.org/0000-0003-1589-832X | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |