Semiconductor-based DNA sequencing of histone modification states
Author(s)
Cheng, Christine S.; Rai, Kunal; Garber, Manuel; Hollinger, Andrew; Robbins, Dana; Anderson, Scott; Macbeth, Alyssa; Tzou, Austin; Carneiro, Mauricio O.; Raychowdhury, Raktima; Russ, Carsten; Hacohen, Nir; Gershenwald, Jeffrey E.; Lennon, Niall; Nusbaum, Chad; Chin, Lynda; Regev, Aviv; Amit, Ido; ... Show more Show less
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Show full item recordAbstract
The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.
Date issued
2013-10Department
Massachusetts Institute of Technology. Department of BiologyJournal
Nature Communications
Publisher
Nature Publishing Group
Citation
Cheng, Christine S. et al. “Semiconductor-Based DNA Sequencing of Histone Modification States.” Nature Communications 4 (2013): n. pag.
Version: Author's final manuscript
ISSN
2041-1723