dc.contributor.author | Cheng, Christine S. | |
dc.contributor.author | Rai, Kunal | |
dc.contributor.author | Garber, Manuel | |
dc.contributor.author | Hollinger, Andrew | |
dc.contributor.author | Robbins, Dana | |
dc.contributor.author | Anderson, Scott | |
dc.contributor.author | Macbeth, Alyssa | |
dc.contributor.author | Tzou, Austin | |
dc.contributor.author | Carneiro, Mauricio O. | |
dc.contributor.author | Raychowdhury, Raktima | |
dc.contributor.author | Russ, Carsten | |
dc.contributor.author | Hacohen, Nir | |
dc.contributor.author | Gershenwald, Jeffrey E. | |
dc.contributor.author | Lennon, Niall | |
dc.contributor.author | Nusbaum, Chad | |
dc.contributor.author | Chin, Lynda | |
dc.contributor.author | Regev, Aviv | |
dc.contributor.author | Amit, Ido | |
dc.date.accessioned | 2014-05-02T14:33:05Z | |
dc.date.available | 2014-05-02T14:33:05Z | |
dc.date.issued | 2013-10 | |
dc.date.submitted | 2013-04 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/86360 | |
dc.description.abstract | The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues. | en_US |
dc.description.sponsorship | National Human Genome Research Institute (U.S.) (1P5)HG006193-01) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Pioneer Award DP1OD003958-01) | en_US |
dc.description.sponsorship | Klarman Cell Observatory | en_US |
dc.language.iso | en_US | |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/ncomms3672 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Semiconductor-based DNA sequencing of histone modification states | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Cheng, Christine S. et al. “Semiconductor-Based DNA Sequencing of Histone Modification States.” Nature Communications 4 (2013): n. pag. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Regev, Aviv | en_US |
dc.relation.journal | Nature Communications | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Cheng, Christine S.; Rai, Kunal; Garber, Manuel; Hollinger, Andrew; Robbins, Dana; Anderson, Scott; Macbeth, Alyssa; Tzou, Austin; Carneiro, Mauricio O.; Raychowdhury, Raktima; Russ, Carsten; Hacohen, Nir; Gershenwald, Jeffrey E.; Lennon, Niall; Nusbaum, Chad; Chin, Lynda; Regev, Aviv; Amit, Ido | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-8567-2049 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |