dc.contributor.author | Bosak, Tanja | |
dc.contributor.author | Klepac-Ceraj, Vanja | |
dc.contributor.author | Tada, Kendall R. | |
dc.contributor.author | Hansel, Colleen M. | |
dc.contributor.author | Ono, Shuhei | |
dc.contributor.author | Prinn, Ronald G. | |
dc.contributor.author | Meredith, Laura Kelsey, 1982- | |
dc.contributor.author | Rao, Deepa,Ph.D.Massachusetts Institute of Technology. | |
dc.date.accessioned | 2015-10-06T18:08:49Z | |
dc.date.available | 2015-10-06T18:08:49Z | |
dc.date.issued | 2014-06 | |
dc.date.submitted | 2013-02 | |
dc.identifier.issn | 17582229 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/99163 | |
dc.description.abstract | Microbe-mediated soil uptake is the largest and most uncertain variable in the budget of atmospheric hydrogen (H[subscript 2]). The diversity and ecophysiological role of soil microorganisms that can consume low atmospheric abundances of H[subscript 2] with high-affinity [NiFe]-hydrogenases is unknown. We expanded the library of atmospheric H[subscript 2]-consuming strains to include four soil Harvard Forest Isolate (HFI) Streptomyces spp., Streptomyces cattleya and Rhodococcus equi by assaying for high-affinity hydrogenase (hhyL) genes and quantifying H[subscript 2] uptake rates. We find that aerial structures (hyphae and spores) are important for Streptomyces H[subscript 2] consumption; uptake was not observed in S. griseoflavus Tu4000 (deficient in aerial structures) and was reduced by physical disruption of Streptomyces sp. HFI8 aerial structures. H[subscript 2] consumption depended on the life cycle stage in developmentally distinct actinobacteria: Streptomyces sp. HFI8 (sporulating) and R. equi (non-sporulating, non-filamentous). Strain HFI8 took up H[subscript 2] only after forming aerial hyphae and sporulating, while R. equi only consumed H[subscript 2] in the late exponential and stationary phase. These observations suggest that conditions favouring H[subscript 2] uptake by actinobacteria are associated with energy and nutrient limitation. Thus, H[subscript 2] may be an important energy source for soil microorganisms inhabiting systems in which nutrients are frequently limited. | en_US |
dc.description.sponsorship | National Science Foundation (U.S.). Graduate Research Fellowship | en_US |
dc.description.sponsorship | Massachusetts Institute of Technology. Undergraduate Research Opportunities Program | en_US |
dc.language.iso | en_US | |
dc.publisher | Wiley Blackwell | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1111/1758-2229.12116 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | Prof. Prinn via Chris Sherratt | en_US |
dc.title | Consumption of atmospheric hydrogen during the life cycle of soil-dwelling actinobacteria | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Meredith, Laura K., Deepa Rao, Tanja Bosak, Vanja Klepac-Ceraj, Kendall R. Tada, Colleen M. Hansel, Shuhei Ono, and Ronald G. Prinn. “Consumption of Atmospheric Hydrogen During the Life Cycle of Soil-Dwelling Actinobacteria.” Environmental Microbiology Reports 6, no. 3 (November 20, 2013): 226–238. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Center for Global Change Science | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences | en_US |
dc.contributor.approver | Prinn, Ronald G. | en_US |
dc.contributor.mitauthor | Meredith, Laura K. | en_US |
dc.contributor.mitauthor | Rao, Deepa | en_US |
dc.contributor.mitauthor | Bosak, Tanja | en_US |
dc.contributor.mitauthor | Ono, Shuhei | en_US |
dc.contributor.mitauthor | Prinn, Ronald G. | en_US |
dc.relation.journal | Environmental Microbiology Reports | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Meredith, Laura K.; Rao, Deepa; Bosak, Tanja; Klepac-Ceraj, Vanja; Tada, Kendall R.; Hansel, Colleen M.; Ono, Shuhei; Prinn, Ronald G. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-5925-3801 | |
dc.identifier.orcid | https://orcid.org/0000-0002-0868-9418 | |
dc.identifier.orcid | https://orcid.org/0000-0001-5179-5323 | |
dc.identifier.orcid | https://orcid.org/0000-0002-1348-9584 | |
dspace.mitauthor.error | true | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |