A Systematic Approach to Identify Candidate Transcription Factors that Control Cell Identity
Author(s)D’Alessio, Ana C.; Fan, Zi Peng; Wert, Katherine J.; Baranov, Petr; Cohen, Malkiel A.; Saini, Janmeet S.; Cohick, Evan; Charniga, Carol; Hannett, Nancy M.; Young, Michael J.; Temple, Sally; Jaenisch, Rudolf; Lee, Tong Ihn; Young, Richard A.; Dadon, Daniel Benjamin; Young, Richard A.; ... Show more Show less
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Hundreds of transcription factors (TFs) are expressed in each cell type, but cell identity can be induced through the activity of just a small number of core TFs. Systematic identification of these core TFs for a wide variety of cell types is currently lacking and would establish a foundation for understanding the transcriptional control of cell identity in development, disease, and cell-based therapy. Here, we describe a computational approach that generates an atlas of candidate core TFs for a broad spectrum of human cells. The potential impact of the atlas was demonstrated via cellular reprogramming efforts where candidate core TFs proved capable of converting human fibroblasts to retinal pigment epithelial-like cells. These results suggest that candidate core TFs from the atlas will prove a useful starting point for studying transcriptional control of cell identity and reprogramming in many human cell types.
DepartmentMassachusetts Institute of Technology. Department of Biology; Whitehead Institute for Biomedical Research
Stem Cell Reports
D’Alessio, Ana C., Zi Peng Fan, Katherine J. Wert, Petr Baranov, Malkiel A. Cohen, Janmeet S. Saini, Evan Cohick, et al. “A Systematic Approach to Identify Candidate Transcription Factors That Control Cell Identity.” Stem Cell Reports 5, no. 5 (November 2015): 763–775.
Final published version