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dc.contributor.authorRobles-Oteiza, Camila
dc.contributor.authorTaylor, Sarah
dc.contributor.authorYates, Travis
dc.contributor.authorCicchini, Michelle
dc.contributor.authorLauderback, Brian
dc.contributor.authorCashman, Christopher R.
dc.contributor.authorBurds, Aurora A.
dc.contributor.authorWinslow, Monte M.
dc.contributor.authorFeldser, David M.
dc.contributor.authorJacks, Tyler E.
dc.date.accessioned2016-01-14T00:28:20Z
dc.date.available2016-01-14T00:28:20Z
dc.date.issued2015-11
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/1721.1/100823
dc.description.abstractSynthetic biological tools that enable precise regulation of gene function within in vivo systems have enormous potential to discern gene function in diverse physiological settings. Here we report the development and characterization of a synthetic gene switch that, when targeted in the mouse germline, enables conditional inactivation, reports gene expression and allows inducible restoration of the targeted gene. Gene inactivation and reporter expression is achieved through Cre-mediated stable inversion of an integrated gene-trap reporter, whereas inducible gene restoration is afforded by Flp-dependent deletion of the inverted gene trap. We validate our approach by targeting the p53 and Rb genes and establishing cell line and in vivo cancer model systems, to study the impact of p53 or Rb inactivation and restoration. We term this allele system XTR, to denote each of the allelic states and the associated expression patterns of the targeted gene: eXpressed (XTR), Trapped (TR) and Restored (R).en_US
dc.language.isoen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/ncomms9783en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleRecombinase-based conditional and reversible gene regulation via XTR allelesen_US
dc.typeArticleen_US
dc.identifier.citationRobles-Oteiza, Camila, Sarah Taylor, Travis Yates, Michelle Cicchini, Brian Lauderback, Christopher R. Cashman, Aurora A. Burds, Monte M. Winslow, Tyler Jacks, and David M. Feldser. “Recombinase-Based Conditional and Reversible Gene Regulation via XTR Alleles.” Nat Comms 6 (November 5, 2015): 8783. © 2015 Macmillan Publishers Limiteden_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorTaylor, Sarahen_US
dc.contributor.mitauthorCashman, Christopher R.en_US
dc.contributor.mitauthorBurds, Aurora A.en_US
dc.contributor.mitauthorJacks, Tyler E.en_US
dc.relation.journalNature Communicationsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsRobles-Oteiza, Camila; Taylor, Sarah; Yates, Travis; Cicchini, Michelle; Lauderback, Brian; Cashman, Christopher R.; Burds, Aurora A.; Winslow, Monte M.; Jacks, Tyler; Feldser, David M.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-5785-8911
mit.licenseOPEN_ACCESS_POLICYen_US


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