Constructive remodeling of a synthetic endothelial extracellular matrix
Author(s)Han, Sewoon; Shin, Yoojin; Jeong, Hyo Eun; Jeon, Jessie S.; Huh, Dongeun; Sohn, Lydia L.; Chung, Seok; Kamm, Roger Dale; ... Show more Show less
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The construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
Nature Publishing Group
Han, Sewoon, Yoojin Shin, Hyo Eun Jeong, Jessie S. Jeon, Roger D. Kamm, Dongeun Huh, Lydia L. Sohn, and Seok Chung. “Constructive Remodeling of a Synthetic Endothelial Extracellular Matrix.” Scientific Reports 5 (December 21, 2015): 18290.
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